综合性中医医院消毒灭菌效果监测与分析.docVIP

综合性中医医院消毒灭菌效果监测与分析 　　摘 要 目的:了解医院临床科室消毒灭菌效果,及时发现问题,分析原因,为控制医院感染提供可靠依据。方法:对全院临床科室的消毒灭菌效果进行抽样监测。结果:2009年使用中的消毒液和高压蒸汽灭菌合格率100%,空气消毒合格率99.36%,物体表面消毒合格率98.14%,医护人员手消毒合格率92.89%。结论:应加强医院消毒灭菌效果的监测,特别是医务人员手卫生的监测,利用监测资料,及时发现医院感染的各个不弱环节并及时整改,降低医院感染的发生率。 　　关键词 综合性中医医院 消毒灭菌 监测 　　doi:10.3969/j.issn.1007-614x.2010.17.158 　　AbstractObjective:to provide reliable evidence for preventing and controlling nosocomial infection,the evaluation of the efficacy of disinfection and sterilization in hospitals clinical departments should be proceeded in a timely manner.Methods:samples of disinfection in each clinical departments of the hospital should be connected.Results:the qualified rate of in-use disinfectant fluids and high pressure steam was 100%,and for the disinfection state of air,the object surface and the hands of medical personnel were 99?36%,98?14% and 92?89% respectively.Conclusions the monitoring of disinfection and sterilization effect should be enhanced,especially that of the hand hygiene of health care workers.By utilizing the monitoring data,the loopholes and weaknesses of the disinfection and sterilization in the hospital can be discovered timely and the accidents can be prevented in time with immediate corrective actions,which could greatly cut down the occurring rate of the hospital infections. 　　Key Wordscomprehensive hospital of Chinese medicine;disinfection and sterilization;monitoring 　　资料与方法 　　2009年对全院24个临床科室的空气、高压蒸汽灭菌效果、使用中的消毒液、医护人员手和物体表面进行监测。 　　采样方法:按照卫生部颁发的《医院感染管理规范》及《消毒技术规范》[1]中规定的医院消毒灭菌效果监测方法进行无菌操作采样;空气监测采用普通琼脂平皿暴露法;使用中消毒液监测用平板倾注法,用一次性无菌空针抽取消毒液1?0ml加入9?0ml含相应中和剂的采样管内混匀,再从管内取上述溶液0?5ml滴入2只灭菌平板内,置36℃温箱培养;高压蒸汽灭菌效果的监测采用生物菌片指示法;医务人员手监测在手消毒后立即采样,用浸有无菌泻脱液的无菌棉拭子在双手指屈面从指根到指端来回涂擦2次,按无菌操作方法剪去手接触部位将棉拭子投入10?0ml的无菌洗脱液试管内,立即送检。物体表面在消毒处理后采样,用含无菌洗脱液的无菌棉拭子,在物体表面涂抹,按无菌操作方法剪去手接触部位将棉拭子投入到10?0ml相应无菌洗脱液采样管中,立即送检。 　　判断标准:根据卫生部颁布的《医院消毒卫生标准》:Ⅱ类房间空气中细菌菌落总数≤200CFU/cm?3,物体表面及医务人员手细菌菌落总数≤5CFU/cm?2,Ⅲ类房间空气中细菌菌落总数≤500CFU/cm?3,物体表面及医护人员手细菌菌落总数≤10CFU/cm?2;使用中消毒液细菌菌落总数≤100CFU/ml;不得检出致病性微生物为