血管紧张素ⅱ及氧化低密度脂蛋白对大鼠血管平滑肌细胞emilin1表达的影响-effects of angiotensin ⅱ and oxidized low density lipoprotein on the expression of emil in 1 in rat vascular smooth muscle cells.docxVIP

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血管紧张素ⅱ及氧化低密度脂蛋白对大鼠血管平滑肌细胞emilin1表达的影响-effects of angiotensin ⅱ and oxidized low density lipoprotein on the expression of emil in 1 in rat vascular smooth muscle cells.docx

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血管紧张素ⅱ及氧化低密度脂蛋白对大鼠血管平滑肌细胞emilin1表达的影响-effects of angiotensin ⅱ and oxidized low density lipoprotein on the expression of emil in 1 in rat vascular smooth muscle cells

三峡大学硕士学位论文三峡大学硕士学位论文 PAGE IV PAGE IV Abstract Objective: This thesis is mainly focusing on different factors influencing the functions of TGF-β, furthermore, to elaborate the mechanism of various pathological changes generated by atherosclerosis and hypertension. A brand-new target for the treatment of atherosclerosis is expected to be found, through full research of the expressional influences upon oxidized low-density lipoprotein, ox-LDL, as well as Angiotensin Ⅱ , Ang Ⅱ towards vascular smooth muscle cells, VSMCs Emilin1 and transforming growth factor-β, TGF-β. Methods: Take 3-10 generation VSMCs in good growth state for culturing, then replaced it with DMEM solution without blood serum (including 0.2% Fetal Bovine Serum) which has been cultured for 24 hours when adherent cells grew up to70%-80% and then kept them synchronized in the quiescent stage. Then they were divided into four groups: blank control group, AngⅡgroup, ox-LDL group, and combined AngⅡand ox-LDL group. After the drug effected for 24 hours, the expression of Emilin1 and TGF-β1 at the level of mRNA and protein were detected by realtime PCR and Western Blot. At last, all the data were analyzed by SPSS13.0 software. Results: The expression of Emilin1 and TGF-β1 for each group : ①the AngⅡ group: after different concentrations of AngⅡaffected, then the expression of Emilin1 decreased (p 0.05), the expression of TGF-β1 increased (p 0.05); ②the ox-LDL group: after different concentrations of ox-LDL were used , the expression of Emilin1 became higher, but the expression of TGF-β1 were lower; ③combined AngⅡand ox-LDL group: the ox-LDL(80mg/L) combined different concentrations of AngⅡ（10-8、10-6、10-4mol/L) used in big mouse. compared with the group of ox-LDL, the expression of Emilin1 was higher(p 0.05), and the expression of TGF-β1 was lower；while compared with the group of blank group, the expression of Emilin1 was obviously higher(p 0.05), and the expression of TGF-β1 was l