c57bl6j小鼠帕金森模型及血浆生物学标记物分析word格式论文.docxVIP

在小鼠模型中诊断PD具有一定准确性。【关键词】帕金森氏病；生物学标记物；正交设计；ROC曲线；模型Parkinson’sdiseasemodelandplasmabiomarkersstudyinC57BL/6jmouseABSTRACT【Abstract】Objective1、Toobservetheeffectofage,drugdosage andadministrationperiodinmousemodelofParkinson’sdisease，in ordertoanalysisthemostimportantfactof thisfourfactsintheonsetofPD，andfindoutan appropriatemodelforPD.2、Usingreceiveroperatingcharacteristic(ROC)curvesanalysistheplasmabiochemical substancesofmousemodelinParkinson’sdiseasetolookforthe appropriate biomarkers which are highly related to the pathologicalchangesanddonoteffectedbyotherfacts.Methods 1、Fourfacts（age,MPTP,probenecid,administrationperiod）aresetupin orthogonaldesigntable L9（34）forgroupingmice.Tyrosine hydroxylaseimmunohistochemistrystaining(TH)positivecellcountingareappliedto indicatethe deficit of dopaminergic neuron in substantia nigra.Swimmingtestandaverageconsumptionoffeedstuffperweek（AFP）are measuredtorevealthe behaviourof mice.2、Four facts（age ,MPTP, probenecid,administrationperiod）aresetupinorthogonaldesigntableL9（34）forgroupingmice.Theplasmabiochemicalsubstancesaretestbyhighefficiencyliquidchromatography(HPLC),colorimetricanalysis andenzyme-linkedimmunosorbentassay.Tyrosinehydroxylase(TH) immunohistochemistry staining positive cell counting are appliedtodraw ROC. Results 1、Administration period is proved the mostimportantfactofmousemodelinPD.Theeffectsequenceofthefactsis- administrationperiodMPTPageprobenecid,andthemost appropriatecombinationisage6months,MPTP 20mg/kg,probenecid200mg/kg,administrationperiod5weeks.Ethologystudyfind swimmingandAFPchangesbothsignificant,withcoefficient correlation0.388、0.450 and P value 0.005、0.0012. 2、ThecoefficientcorrelationsbetweenTHCPCandDA,Ach,8-OHdG,UA,EGFare 0.07,0.05,-0.67,0.23,0.68,withPvalue0.000、0.110、0.7150、000、0.654.Andtheareaundercurve(AUC)areseparately0.53,0.53,0.82,0.55,0.82.Conclusions1、AdministrationperiodisprovedthemostimportantfactofmousemodelinPD.Itcanprobablybeusedtoreducethedrugdosage. 2、TheAUCof8-Ohd