c.jejuni经km雄鼠消化道连续传代后形态 毒力因子致周围神经病能力的变化研究word格式论文.docxVIP

目录中文摘要············································································································1 英文摘要············································································································3 英文缩写············································································································6 研究论文 C.jejuni 经 KM 雄鼠消化道连续传代后形态、毒力因子致周围神经病能力的变化研究前言············································································································7 材料与方法································································································8 结果··········································································································20 附图··········································································································23 附表··········································································································27 讨论··········································································································32 结论··········································································································36 参考文献··································································································37 综述 空肠弯曲菌分型方法的研究进展························································39 致谢··················································································································48 个人简历··········································································································49C.jejuni 经 KM 雄鼠消化道连续传代后形态、毒力因子致周 围神经病能力的变化研究摘要目的：研究空肠弯曲菌（C.jejuni）经 KM 雄鼠消化道连续传代分离 后，细菌的形态学、生长速度和运动能力等相关毒力因子致周围神经病能 力的变化。以期获得毒力增强且稳定遗传的 C.jejuni 菌株，提高 C.jejuni 致周围神经病动物模型的发病率，进一步改良此模型，同时也为研究 C.jejuni 致周围神经病致病机理提供实验数据。方法：复苏 C.jejuni lulei 株，多种方法鉴定后，将 P4 代作为攻毒菌株并制 成 3×108cfu/ml 菌悬液，经口灌胃攻毒 4 周龄清洁级昆明（KM）雄鼠， 分别取 24 小时、48 小时粪便分离培养 C.jejuni 分离株，用得到的新一代 24 小时分离菌株继续攻毒新一批小鼠，照此方法连续传代十八代。通过 对小鼠一般情况的观察，对每代不同时间点分离株进行形态学对比，生长 速度测定以及细菌动