三种高密度脂蛋白胆固醇检验方法的比较.docVIP

三种高密度脂蛋白胆固醇检验方法的比较 　　[摘要]目的 ?Ρ确治龈呙芏戎?蛋白胆固醇（HDL-C）的检验中聚阴离子多聚物/表面活性剂（PPD）法和PEG修饰酶/硫酸α-环糊精（PEGME）法及沉淀法的准确性和特异性。方法 分别将已知浓度为1.0～2.0、2.0～4.0 mmol/L的HDL-C、LDL-C在生化分析仪上进行测试，运用两点终点法对参数进行分析，主、辅波长分别为600、700 nm，在3 μl标本量中加入240 μl R1，288 s后读取吸光度A1，反应5 min后再将80 μl R2加入其中，600 s后读取吸光度A2。依据参考品的HDL-C、LDL-C浓度值计算其含量。结果 PPD法试剂对HDL-C具有相对缓慢的作用，对LDL的非特异性反应较为显著，达到10%～20%；PEGME法试剂对HDL-C具有相对较快的作用，对LDL-C基本不具有非特异性反应，仅为5%以下。HDL-C、LDL-C、Trig的正常值分别为1.1～1.7、1.7～2.9、0.4～1.8 mmol/L，PPD法的结果高于沉淀法7%～15%。将参照设定为沉淀法结果，如果血清标本在正常胆红素浓度范围内，则PPD法测定结果就具有假性增高的表现，而如果血清标本胆红素浓度20 μmol/L，则PPD法测定结果就具有明显降低的表现，达到参照值的60%；胆红素浓度变化极少干扰PEGME法测定结果。将参照设定为沉淀法结果，PPD法测定结果受到三酰甘油的影响显著提升了20%以上，而三酰甘油浓度的提升使其进一步假性增高；PEGME法较少受到三酰甘油的影响。两者均与三酰甘油浓度成负性关系。PPD法的结果显著高于沉淀法（t=4.303，P 　　[关键词]高密度值蛋白胆固醇；沉淀法；聚阴离子多聚物/表面活性剂法；PEG修饰酶/硫酸α-环糊精法 　　[中图分类号] R446.11+2 [文献标识码] A [文章编号] 1674-4721（2016）12（a）-0148-04 　　Comparative analysis of three test methods of high density protein cholesterol 　　ZOU Shu-qin 　　Clinical Laboratory，People′s Hospital of Fengshun County in Meizhou City，Guangdong Province，Fengshun 514300，China 　　[Abstract]Objective To compare and analyze the accuracy and specificity of polyanionic polymer/surfactant （PPD） method and PEG-modified enzyme/sulfate α-cyclodextrin method （PEGME） and precipitation method in high-density lipoprotein cholesterol （HDL-C） inspection.Methods The known concentration 1.0 to 2.0，2.0 to 4.0 mmol/L of HDL-C，LDL-C were tested on biochemical analyzer，the parameters were analyzed by two point end method，the primary and secondary wavelength were 600 nm，700 nm，respectively，240 μl R1 were joint to 3 μl sample，288 s absorbance A1 volume was readed out，the reaction for 5 min before the 80 μl R2 added，600 s absorbance A2 after reading it.values were calculated its content according to the reference product of HDL-C，LDL-C concentration.Results PPD reagent had relatively slow effect on HDL-C，nonspecific response to LDL-C was up to 10% to 20%；PEGME reagent had relatively high effect on HDL-C，the LDL-C did not have a nonsp