脂氧素A4对幼年肥胖大鼠血管内皮细胞增殖的影响.docVIP

精品论文 参考文献 脂氧素A4对幼年肥胖大鼠血管内皮细胞增殖的影响 徐州医科大学附属医院儿科 江苏徐州 221002 【摘 要】目的 通过检测脂氧素A4（LXA4）作用于幼年肥胖SD大鼠内皮细胞前后内皮细胞增殖变化，探讨LXA4在改善幼年肥胖大鼠内皮细胞损伤中的可能作用。方法 40只三周龄断乳的雄性SD大鼠采用随机数字表法随机分为高脂组（HF组 n=32）与对照组（NC组 n=8）。对照组给予普通饲料，高脂组给予高脂饲料，饲养6周后，用贴块法培养内皮细胞，用MTT比色法检测不同浓度LXA4（0ug/ L、1ug/ L、10ug/L、100ug/ L）作用于原代培养的肥胖大鼠内皮细胞24h后的增殖情况，测定OD值。结果 不同浓度LXA4（0ug/ L、1ug/ L、10ug/L、100ug/ L）作用于原代培养肥胖大鼠内皮细胞24h后，可抑制其增殖，OD值显著降低，且呈浓度依赖性，对照组与实验组，实验组组间比较，均有统计学差异（Plt;0.01）。结论 LXA4可抑制肥胖大鼠内皮细胞增殖。 【关键词】肥胖；脂氧素A4；血管内皮细胞 Effect of Lipoxin A4 on proliferation of endothelial cells in childhood obesity rats Zhao Tong，Wu xiaondash;lu，Gao li-li.Department of pediatrics，Affiliated Hospital of Xu Zhou Medical Collage，Xu Zhou 221002，China. Abstract：Object To investigate the effect of LipoxinA4 on proliferation in vascular endothelial cell of young obesity rat．To investigate the role of LipoxinA4 may play on improving vascular endothelial cell dysfunction of young obesity rat.thus to provide some new ideas for preventing and treating of obesity and cardiovascular diseases.Methods Forty three-week old male SD weaning rats were randomly divided into two groups，the high fat group（n=32）and the control group（n=8）.And they were fed standard diet and a high fat diet.After six weeks endothelial cells of the obesity rats were cultured in vitro，the effect of different concentration of lipoxinA4（0、1、10and100ug/L）on primary cultured obesity rat endothelial cells proliferation was assessed by MTT colorimetry after 24 hours，and then determine their OD mV.Results Proliferation of primary cultured rat vascular endothelial cell was promoted by lipoxinA4（0、1、10and100ug/L）after 24 hours.Proliferation of endothelial cell was suppressed by lipoxinA4，significant difference was found in between the experimental groups and the control groups according to statistics，Plt;0.01．Conclusion LipoxinA4 inhibits proliferation of primary cultured obesity rat vascular endothelial cell. Key words：obesity；lipoxin A4；vascular endothelial cells 【中图分类号】R891+.