丹参注射液对糖尿病肾病大鼠肾脏线粒体呼吸及能量代谢的影响.docVIP

精品论文 参考文献 丹参注射液对糖尿病肾病大鼠肾脏线粒体呼吸及能量代谢的影响 王晓华1 欧阳玉林2 何杏容1 黄华庆1 赵玉珠1 (1广州市番禺区何贤纪念医院 511400; 2广州市番禺区中心医院 511400) 【摘要】目的：探讨丹参对糖尿病肾病（DN）大鼠肾脏功能及线粒体呼吸和能量代谢的影响。方法：选择健康SD大鼠24只，按随机数字表法分为DN模型组（DN组）、丹参注射液干预组（丹参组）和正常对照组（对照组），每组各8只。DN组及丹参组大鼠使用链脲佐菌素制作DN大鼠模型，丹参组加予丹参注射液灌胃，正常组和DN组同时给予等量的生理盐水，连续喂养6周。检测空腹血糖、血尿素氮、血清肌酐、24小时尿量及尿蛋白定量，留取肾组织，高效液相色谱法检测ATP、ADP、AMP，提取线粒体，氧电极法检测线粒体3态呼吸速率（T3）、4态呼吸速率（T4）和呼吸控制率(RCR)。结果：与DN组大鼠比较，丹参组大鼠的血糖水平、血尿素氮、血清肌酐、尿蛋白定量下降，24 h尿量增加，肾脏组织线粒体T3和RCR升高，ATP、ADP含量增加，P均lt;0.05。结论：丹参可能通过改善DN大鼠肾脏线粒体呼吸功能及能量代谢，改善DN大鼠肾脏的功能。 【关键词】丹参 糖尿病肾病 线粒体 能量代谢 【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2013）26-0163-03 Protect effect of Salvia miltiorrhiza on renal mitochondrial energy metabolism in rats with diabetic nephropathy Wang Xiao-hua.，Ouyang yulin , He xingrong , Huang huaqing , Zhao yuzhu， Department of Nephrology, He Xian Memorial Hospital of Panyu District, Guangzhou, 511400 【Abstract】Objective: To investigate the effects of Salvia miltiorrhiza on the mitochondrial respiration and energy metabolism in diabetic nephropathy rat. Methods: 24 healthy SD rats were randomly divided into DN group , Salvia miltiorrhiza group and Normal group.Each group has 8 rats. DN group and Salvia miltiorrhiza group were induced by STZ. Salvia miltiorrhiza group were given Salvia miltiorrhiza per day, while the DN group and Normal group were given Normal saline respectively for 6 weeks.The level of serum glucose , BUN , Cr and 24-hour urine albumin and urine volume were examined. HPLC was used to evaluate the content of ATP，ADP and AMP.Mitochondria were isolated with different centrifugations，the Clark electrodes were used to test the mitochondrial T3，T4 and RCR. Results:Compared with DN group, the serum glucose , BUN , Cr , 24-hour urine albumin were lower in Salvia miltiorrhiza group . The 24-hour urine volume , the content of ATP and ADP of kidney , the mitochondrial T3 and RCR were higher in Salvia miltiorrhiza group than those in DN gro