促红素对脑缺血再灌注炎症损伤的抑制作用研究.docVIP

精品论文 参考文献 促红素对脑缺血再灌注炎症损伤的抑制作用研究 赵德福1 宁显忠1 王阿楠2 　　（1.辽宁医学院附属第三医院 神经内科 辽宁 锦州 121000；2.辽宁医学院附属第三医院 重症监护病房 辽宁 锦州 121000） 　　【摘 要】目的：观察促红细胞生成素在局灶性脑缺血再灌注模型大鼠中对核因子-kB、环氧化酶2表达的影响，探讨EPO对大鼠脑缺血再灌注后炎症反应的抑制作用。方法：SD大鼠随机分为假手术组（SH）、模型/缺血再灌注组（IR）、EPO处理组（EPO）、二硫代氨基甲酸吡咯烷（PDTC）处理组（PDTC）和PDTC+EPO组（PDTC+EPO）。相应处理后，各组行神经功能缺损评分，标本用于免疫组织化学和Western Blot分析海马组织NF-kappa;B、COX-2的表达。结果：与IR组相比，其余各处理组大鼠神经功能缺损评分均明显下降（Plt;0.01），NF-kappa;B及COX-2表达均明显减少（Plt;0.01），PDTC组COX-2的表达仍较SH组偏高（Plt;0.01）；与PDTC组比较，EPO组和PDTC+EPO组大鼠神经功能缺损评分均明显下降（Plt;0.01），COX-2的表达明显减少（Plt;0.01）；而EPO组和PDTC+EPO组之间大鼠神经功能缺损评分及COX-2的表达差异无统计学意义（Pgt;0.05）。结论：EPO通过两条途径降低缺血侧海马COX-2蛋白表达减轻脑缺血再灌注大鼠海马缺血区的炎症反应，从而发挥其神经保护功能。 　　【关键词】促红细胞生成素；脑缺血再灌注；核因子-kappa;B；环氧化酶2 　　【中图分类号】R543 【文献标识码】A 【文章编号】1004-6194（2015）01-0111-03 　　Stuty on the inhibition of erythropoietin to inflammation of focal cerebral ischemia-reperfusion injury in rats 　　Zhao Defu1 Ning Xianzhong1 Wang Anan2 　　（1.Department of Neurology, Third Affiliated Hospital, Liaoning Medical University, Jinzhou 121000） 　　（2. ICU of Neurology, Third Affiliated Hospital, Liaoning Medical University, Jinzhou 121000） 　　【Abstract】Objective：To investigate the influence of EPO on the expression of NF-kappa;B, cox-2 with focal cerebral ischemia-reperfusion ; To explore the inhibition of EPO to inflammation with focal cerebral ischemia-reperfusion injury in rats. Methods：SD rats were randomly divided into 5 groups : SH, IR, PTDC, EPO, PDTC+EPO group. Rats made into the modal were Observed the neurological scores. The brain tissues of rats were observed by immunohistochemical staining and Western Blot. Results：Contrast to the IR group, the scores of the other rats were decreased (Plt;0.01); the expression of NF-kappa;B and cox-2 were decreased obviously (Plt;0.01). The expression of cox-2 in PDTC group was higher than SH group (Plt;0.01). Contrast to the PDTC group, the scores in EPO and PDTC+EPO group were decreased (Plt;0.01); the expression of cox-2 were decreased obviously (Plt;0.01).