利用叶绿体基因组高变片段对7个软枣猕猴桃居群遗传多样性的研究.pdfVIP

Botanical Research 植物学研究, 2014, 3, 238-248 Published Online November 2014 in Hans. /journal/br /10.12677/br.2014.36030 Study on Genetic Diversity of 7 Populations of Actinidia by Using Chloroplast Genome Fragment * Hong Liu , Xihong Liu, Qiuyu Liu, Gang Li, Rui Qin Engineering Research Center for Protection and Utilization of Bioresource in Ethnic Area of Southern China, South-Central University for Nationalities, Wuhan * Email: liuhong@ th th th Received: Sep. 14 , 2014; revised: Oct. 16 , 2014; accepted: Oct. 27 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Actinidia arguta is one of the most complex species in the genus of Actinidia with different ploidy. In this paper, we choose seven representative wild populations of A. arguta in four provinces of China. We use 21 pairs of chloroplast genome hypervariable segments to analyse genetic diversity of seven populations. The result shows that seven pairs within twenty one pairs of primer frag- ment can amplify samples successfully. The No. 13 fragments rpl32-trnL had the best resolution ratio. We used the fragment rpl32-trnL to build the phylogenetic tree of seven populations with MP algorithm and NJ method. The experimental results show that two populations from Changbai Mountain are located at the base of the tree. Three populations from the Dabie Mountains are not in the same branch. At last, the average polymorphic locus of 21 fragments is 27.16. This study provided basis for the development and application of c