乳腺癌组织中COX-2及IL-8表达相关性及临床意义.docVIP

乳腺癌组织中COX-2及IL-8表达相关性及临床意义 　 作者：陈守华　张丽丽　顾禾　付荣湛 【摘要】 目的：探讨乳腺癌组织中环氧化酶-2（COX-2）和白细胞介素-8（IL-8）表达的相关性及临床意义。方法：采用免疫组织化学法对60例乳腺癌患者病理组织中的COX-2和IL-8进行检测，并与31例乳腺良性疾病对照，分析其相关性，以及二者与肿瘤大小、浸润程度、TNM分期、淋巴结转移及雌激素受体（estrogen receptor， ER）和孕激素受体（progesteronreceptor，PR）表达等生物学行为的关系。 结果：乳腺癌组织中COX-2和IL-8阳性表达率分别为66.7%、60.0%均显著高于对照组（P=0.000 6、P=0.000 2），且二者表达存在明显正相关（r=0.433，Plt;0.001）。COX-2和IL-8阳性细胞表达率与肿瘤大小、浸润程度、TNM分期、淋巴结转移情况均有明显差异（P均lt; 0.05），而与ER和PR的表达状态无明显相关（P均gt;0.05）。结论： COX-2和IL-8在乳腺癌组织中过度表达且呈正相关，提示乳腺癌组织中COX-2和IL-8存在相互调控机制，共同促进肿瘤的发生和发展。 【关键词】 乳腺肿瘤·环氧化酶-2·白细胞介素-8·免疫组织化学 【ABSTRACT】Objective：To study the relationship and significance of the expression of COX-2 and IL-8 in cells of breast cancer. Methods：Speciments from 60 patients with breast cancer and 31 patients with benign breast diseases were studied.Agiogene was assessed by the expression of cyclooxygenase-2(COX-2) and interleukin-8(IL-8) using immunohistochemical staining, study the relationship, as to analyze relationships between them and tumor size,tumor type,TNM stages, lymph nodes metastasis and expression of estrogen receptor(ER) and progestin receptor（PR）as well.Results：The expression rate of COX-2 and IL-8 in breast cancer cells were higher than that of normal controls(66.7%、60.0%；P=0.0006、 P=0.0002).The expression rate of IL-8 in breast cancer of positive expression of COX-2 were higher(r=0.433、Plt;0.001).There were significant correlations between COX-2、IL-8 and tumor size，tumor type，TNM stages and lymph node metastasis(Plt;0.05), but non-significant correlations with ER、PR（Pgt;0.05）. Conclusion：The high expression of COX-2 and IL-8 in cells of breast cancer was positive correlated.,suggesting they may regulate each other and jointly contribute to the tumorigenesis and progression of breast cancer. 【KEY WORDS】 Breast tumor·COX-2·IL-8·Immunohistochemistry 　　乳腺癌是女性最常见的恶性肿瘤之一，乳腺癌发生转移是患者死亡的重要原因。有文献报道，在乳腺癌的浸润转移过程中，COX-2和IL-8具有重要作用［１-3］。本研究采用免疫组织化学法检测乳腺癌组织中COX-2和IL-8的表达情况，进一步探讨两者之间的关系及临床意义。 1　资料与方法 1.1　一般资料　乳腺癌60例均为山东省千佛