乳腺癌组织中VEGF及C-erbB-2表达及意义.docVIP

乳腺癌组织中VEGF及C-erbB-2表达及意义 　 作者：粟连秀，张兆明，陈红秋，杨达平，李雄 【摘要】 目的 探讨乳腺癌组织中血管内皮细胞生长因子（VEGF）及原癌基因C-erbB-2的表达及其与临床病理特征之间的关系。方法 采用快捷免疫组化MaxVisionTM法，对80例乳腺癌组织和20例乳腺良性病变组织中VEGF及C-erbB-2的表达进行检测。结果 VEGF与C-erbB-2在乳腺癌的阳性表达率分别为62.50%和35.00%，高于在乳腺良性病变组织中的零表达(P均＜0.01)。VEGF和C-erbB-2的阳性表达与乳腺癌患者的年龄、肿瘤大小和组织学分型无关（Pgt;均0.05），而与腋窝淋巴结转移有关（P＜0.05或0.01)，VEGF还与临床分期有关（P＜0.05)。乳腺癌中VEGF表达与C-erbB-2表达密切有关（χ2＝12.97，P＜0.01）。结论 VEGF和C-erbB-2参与了乳腺癌的发生和发展过程，VEGF和C-erbB-2可作为临床上判定乳腺癌预后的指标。 【关键词】 乳腺肿瘤；VEGF；C-erbB-2；免疫组织化学 　　Abstract: Objective To outline the expressions of vascular endothelial growth factor (VEGF) in breast cancer tissues and initial cancer gene C-erbB-2 as well as their relationship with clinical pathological characteristics. Methods A MaxVisionTM method was used to detect the expression of VEGF and C-erbB-2 in 80 patients with breast cancer and 20 specimen of breast benign lesion tissues. Results 62.50% patients were found with positive expression of VEGF and 35.00% patients with positive expression of C-erbB-2 in breast cancer tissues, in comparison to the benign lesion tissues (no positive expression was found), there were statistically significant differences (Plt;0.01). The expression of VEGF and C-erbB-2 had no correlation with the breast cancer patients? age, tumor size and histological classification (Pgt;0.05), and correlated positively with the lymph node metastasis (Plt;0.05 or 0.01), VEGF correlated positively with clinical stage (Plt;0.05). The expression of VEGF in breast cancer correlated positively with the expression of C-erbB-2 (χ2=12.97,Plt;0.01). Conclusion VEGF and C-erbB-2 play an important role in the oncogenesis and development of breast cancer, VEGF and C-erbB-2 may be markers for clinically predicting the prognosis of breast cancer. 　　Key words: breast neoplasm; VEGF; C-erbB-2; immunohistochemistry 　　乳腺癌是危害妇女健康的常见恶性肿瘤之一，其发病率和病死率均呈较快的增长趋势。乳腺癌与其它肿瘤一样，肿瘤的发生、发展及预后与多种因素有关。本组研究应用免疫组化方法检测80例乳腺癌中血管内皮细胞生长因子(VEGF)、原癌基因(C-erbB-2)的表达，探讨乳腺癌组织中VEGF和C-erbB-2的表达情况及其与临床病理特征之间的关系