β-氨基丙腈抑制赖氨酰氧化酶对小鼠免疫功能影响.docVIP

β-氨基丙腈抑制赖氨酰氧化酶对小鼠免疫功能影响 　【摘要】 目的 观察β-氨基丙腈(β-Aminopropionitrile，BAPN)抑制赖氨酰氧化酶(lysyl oxidase，LOX)后小鼠免疫功能的变化。方法 选用18～22g的正常ICR小鼠，以β-氨基丙腈100mg/kg连续灌胃10d。处死后检测小鼠胸腺重、脾重、巨噬细胞吞噬功能；采用绵羊红细胞作为抗原，检测小鼠抗体生成细胞功能；采用MTT法检测小鼠脾淋巴细胞对ConA刺激的转化能力。结果 以β-氨基丙腈抑制LOX活性，明显降低小鼠胸腺/体重比值；巨噬细胞吞噬率及吞噬指数均下降；小鼠抗体生成细胞数减少；ConA诱导的小鼠脾淋巴细胞增殖能力减弱。结论 β-氨基丙腈抑制赖氨酰氧化酶可减弱小鼠免疫功能。 【关键词】 赖氨酰氧化酶；β-氨基丙腈；免疫功能；小鼠 　　Abstract: Objective To explore the changes of immune function in mice after lysyl oxidase (LOX) was inhibited by β-Aminopropionitrile (BAPN). Methods Normal ICR mice with weight of 18～22 grams were divided into two groups. The experimental group was intragastric administration of BAPN with dose 100mg/kg for 10 days. After killing the mice, weights of thymuses and spleens, and the macrophage phagocytosis were detected. Using sheep red blood cells as antigen, the amounts of antibody-producing cells in mice were counted. Through lymphocyte conversion test, lymphocyte conversion ratios of spleen lymphocytes stimulated by ConA in mice were determined. Results After LOX activities were inhibited by BAPN, the thymus/ body weight ratios of mice were reduced significantly in experimental group. The phagocytic rates and phagocytic indexes of macrophages were significantly decreased. The numbers of antibody-producing cells declined. In the meanwhile, con A-induced lymphocyte conversion ratios of mice spleen lymphocytes were weakened. Conclusion Inhibition of LOX by BAPN can decrease the immune function in mice. 　　Key words: lysyl oxidase； β-Aminopropionitrile；immune function；mice 　　赖氨酰氧化酶(lysyl oxidase，LOX)是一种细胞外依赖铜的氨基酸氧化酶，参与细胞外基质中胶原和弹性蛋白赖氨酸残基交联，在胶原和弹性蛋白由可溶性单体转变为不溶性纤维过程的初始阶段发挥重要作用［1］，对维持细胞外基质的结构和功能正常、组织器官发育、创伤修复、细胞运动等都具有重要意义［2］。β-氨基丙腈(β-Aminopropionitrile，BAPN)能抑制胶原交联的形成，是LOX不可逆的抑制剂［3］。本文以BAPN抑制LOX，进而从非特异免疫、体液免疫、细胞免疫三个方面初步研究小鼠免疫功能的变化。 　　1 材料与方法 　　1.1 材料 　　BAPN、刀豆蛋白A(ConA)和四甲基偶氮唑蓝(MTT)均为美国SIGM公司产品(购自北京华美生物科技有限公司、北京思语生物科技有限公司)；胎牛血清(购自北京拜尔迪生物科技有限公司)；RPMI-1640培养基为美国GIBCO公司产品(购自北京拜尔迪生物科技有限公司)；选用宁夏医学院实验动物中心提供的清洁级ICR小鼠。 　　1.