xmeis3 is necessary for mesodermal hox gene expression and functionxmeis3是中胚层hox基因表达和功能所必需的.pdfVIP

XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function 1 2 2 Paul M. J. In der Rieden , Hans J. Jansen , Antony J. Durston * 1 Studiekring, Utrecht, Netherlands, 2 Sylvius Laboratory, Leiden, Netherlands Abstract Hox transcription factors provide positional information during patterning of the anteroposterior axis. Hox transcription factors can co-operatively bind with PBC-class co-factors, enhancing specificity and affinity for their appropriate binding sites. The nuclear localisation of these co-factors is regulated by the Meis-class of homeodomain proteins. During development of the zebrafish hindbrain, Meis3 has previously been shown to synergise with Hoxb1 in the autoregulation of Hoxb1. In Xenopus XMeis3 posteriorises the embryo upon ectopic expression. Recently, an early temporally collinear expression sequence of Hox genes was detected in Xenopus gastrula mesoderm (see intro. P3). There is evidence that this sequence sets up the embryo’s later axial Hox expression pattern by time-space translation. We investigated whether XMeis3 is involved in regulation of this early mesodermal Hox gene expression. Here, we present evidence that XMeis3 is necessary for expression of Hoxd1, Hoxb4 and Hoxc6 in mesoderm during gastrulation. In addition, we show that XMeis3 function is necessary for the progression of gastrulation. Finally, we present evidence for synergy between XMeis3 and Hoxd1 in Hoxd1 autoregulation in mesoderm during gastrulation. Citation: In der Rieden PMJ, Jansen HJ, Durston AJ (2011) XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function. PLoS ONE 6(3): e18010. doi:10.1371/journal.pone.0018010 Editor: Christoph Winkler, Natio