wheat drought-responsive grain proteome analysis by linear and nonlinear 2-de and maldi-tof mass spectrometry小麦drought-responsive谷物的蛋白质组分析线性和非线性两种和maldi-tof质谱分析.pdfVIP
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wheat drought-responsive grain proteome analysis by linear and nonlinear 2-de and maldi-tof mass spectrometry小麦drought-responsive谷物的蛋白质组分析线性和非线性两种和maldi-tof质谱分析
Int. J. Mol. Sci. 2012, 13, 16065-16083; doi:10.3390/ijms131216065 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Wheat Drought-Responsive Grain Proteome Analysis by Linear and Nonlinear 2-DE and MALDI-TOF Mass Spectrometry Shan-Shan Jiang 1,†, Xiao-Na Liang 1,†, Xin Li 1,†, Shun-Li Wang 1,†, Dong-Wen Lv 1, Chao-Ying Ma 1, Xiao-Hui Li 1, Wu-Jun Ma 2,* and Yue-Ming Yan 1,* 1 College of Life Science, Capital Normal University, Beijing 100048, China; E-Mails: jianghuan163.com (S.-S.J.); liangxiaona2008@126.com (X.-N.L.); xin.li@ (X.L.); shunliky@163.com (S.-L.W.); ldwswjsn1@126.com (D.-W.L.); machao3721@ (C.-Y.M.); lixiaohui1978@163.com (X.-H.L.) 2 State Agriculture Biotechnology Centre, Western Australian Department of Agriculture and Food, Perth, WA 6150, Australia † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: w.ma@M.au (W.-J.M.); yanym@ (Y.-M.Y.); Tel./Fax : +61-8(W.-J.M.); Tel./Fax: +86-10(Y.-M.Y.). Received: 15 October 2012; in revised form: 13 November 2012 / Accepted: 14 November 2012 / Published: 29 November 2012 Abstract: A comparative proteomic analysis of drought-responsive proteins during grain development of two wheat varieties Kauz (strong resistance to drought stress) and Janz (sensitive to drought stress) was performed by using linear and nonlinear 2-DE and MALDI-TOF mass
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