yeast transcription termination factor rtt103 functions in dna damage response酵母转录终止因子rtt103函数在dna损伤反应.pdfVIP

Yeast Transcription Termination Factor Rtt103 Functions in DNA Damage Response Indukuri Srividya, Sirupangi Tirupataiah, Krishnaveni Mishra* Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India Abstract YKu70/YKu80 is a heterodimer that is essential for repair of DNA double strand breaks through non-homologous end joining pathway in the yeast Saccharomyces cerevisiae. Yku70/80 proteins are associated with telomeres and are important for maintaining the integrity of telomeres. These proteins protect telomeres from recombination events, nuclease attacks, support the formation of heterochromatin at telomeres and anchor telomeres to the nuclear periphery. To identify components in molecular networks involved in the multiple functions of Yku70/80 complex, we performed a genetic screen for suppressors of yku70 deletion. One of the suppressors identified was RTT103, which encodes a protein implicated in transcription termination. We show that rtt103D are sensitive to multiple forms of genome insults and that RTT103 is essential for recovery from DNA double strand breaks in the chromosome. We further show that Rtt103 associates with sites of DNA breaks and hence is likely to play a direct role in response to DNA damage. Citation: Srividya I, Tirupataiah S, Mishra K (2012) Yeast Transcription Termination Factor Rtt103 Functions in DNA Damage Response. PLoS ONE 7(2): e31288. doi:10.1371/journal.pone.0031288 Editor: Arthur J. Lustig, Tulane University Health Sciences Center, United States of America Received October 3, 2011; Accepted January 5, 2012; Published February 15, 2012 Copyright: 2012 Srividya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted