whole genome sequencing to investigate the emergence of clonal complex 23 neisseria meningitidis serogroup y disease in the united states全基因组测序研究克隆的出现复杂23脑膜炎奈瑟菌血清组y疾病在美国.pdfVIP

Whole Genome Sequencing to Investigate the Emergence of Clonal Complex 23 Neisseria meningitidis Serogroup Y Disease in the United States 1 2 2 2 1 Mary G. Krauland *, Julie C. Dunning Hotopp , David R. Riley , Sean C. Daugherty , Jane W. Marsh , 3 3 ´ 2 1 Nancy E. Messonnier , Leonard W. Mayer , Herve Tettelin , Lee H. Harrison 1 Infectious Diseases Epidemiology Research Unit, School of Medicine and Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvanian, United States of America, 2 The Institute for Genome Sciences, University of Maryland School of Medicine, University of Maryland, Baltimore, Maryland, United States of America, 3 Meningitis and Vaccine Preventable Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America Abstract In the United States, serogroup Y, ST-23 clonal complex Neisseria meningitidis was responsible for an increase in meningococcal disease incidence during the 1990s. This increase was accompanied by antigenic shift of three outer membrane proteins, with a decrease in the population that predominated in the early 1990s as a different population emerged later in that decade. To understand factors that may have been responsible for the emergence of serogroup Y disease, we used whole genome pyrosequencing to investigate genetic differences between isolates from early and late N. meningitidis populations, obtained from meningococcal disease cases in Maryland in the 1990s. The genomes of isolates from the early and late populations were highly similar, with 1231 of 1776 shared genes