whole-blood flow-cytometric analysis of antigen-specific cd4 t-cell cytokine profiles distinguishes active tuberculosis from non-active states遗传flow-cytometric cd4 t细胞抗原细胞因子分析概要文件区分活动性结核病与稳定的状态.pdfVIP

Whole-Blood Flow-Cytometric Analysis of Antigen- Specific CD4 T-Cell Cytokine Profiles Distinguishes Active Tuberculosis from Non-Active States 1 2 2 3¤ 4 5 6 Urban Sester , Mathias Fousse , Jan Dirks , Ulrich Mack , Antje Prasse , Mahavir Singh , Ajit Lalvani , Martina Sester2* 1 Department of Internal Medicine IV, Saarland University, Homburg, Germany, 2 Department of Transplant and Infection Immunology, Institute of Virology, Saarland University, Homburg, Germany, 3 Department of Internal Medicine V, Saarland University, Homburg, Germany, 4 Department of Pneumology, University Medical Center Freiburg, Freiburg, Germany, 5 Helmholtz Center for Infection Research, and Lionex Diagnostics Therapeutics GmbH, Braunschweig, Germany, 6 Tuberculosis Research Unit, Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London, London, United Kingdom Abstract T-cell based IFN-c release assays do not permit distinction of active tuberculosis (TB) from successfully treated disease or latent M. tuberculosis infection. We postulated that IFN-c and IL-2 cytokine profiles of antigen-specific T cells measured by flow-cytometry ex vivo might correlate with TB disease activity in vivo. Tuberculin (PPD), ESAT-6 and CFP-10 were used as stimuli to determine antigen-specific cytokine profiles in CD4 T cells from 24 patients with active TB and 28 patients with successfully treated TB using flow-cytometry. Moreover, 25 individuals with immunity consistent with latent M. tuberculosis infection and BCG-vaccination, respectively, were recruited. Although the frequency of cytokine secreting PPD reacti