antiviral activity and mechanism of action of novel thiourea containing chiral phosphonate on tobacco mosaic virus抗病毒活性和新颖的含手性硫脲的作用机制对烟草花叶病毒膦酸酯.pdfVIP
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antiviral activity and mechanism of action of novel thiourea containing chiral phosphonate on tobacco mosaic virus抗病毒活性和新颖的含手性硫脲的作用机制对烟草花叶病毒膦酸酯
Int. J. Mol. Sci. 2011, 12, 4522-4535; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Antiviral Activity and Mechanism of Action of Novel Thiourea
Containing Chiral Phosphonate on Tobacco Mosaic Virus
Huitao Fan, Baoan Song *, Pinaki S. Bhadury, Linhong Jin, Deyu Hu and Song Yang *
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key
Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou
University, Guiyang 550025, China; E-Mails: fanhuitao0818@163.com (H.F.);
bhadury@ (B.P.); fcc.jinlh@ (L.J.); fcc.dyhu@ (D.H.)
* Authors to whom correspondence should be addressed; E-Mails: songbaoan22@ (B.S.);
gdjhzx@ (S.Y.); Tel.: +86-851-362-0521; Fax: +86-851-829-2170.
Received: 30 May 2011; in revised form: 18 June 2011 / Accepted: 4 July 2011 /
Published: 13 July 2011
Abstract: Using half-leaf method O,O’-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3-
phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its
activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the
curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control
agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition
rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of
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