an integrated approach to elucidate the intra-viral and viral-cellular protein interaction networks of a gamma-herpesvirus一个集成的方法来阐明intra-viral gamma-herpesvirus和viral-cellular蛋白质相互作用网络.pdfVIP

An Integrated Approach to Elucidate the Intra-Viral and Viral-Cellular Protein Interaction Networks of a Gamma- Herpesvirus 1,2. 3. 4 5 5 Shaoying Lee , Lukasz Salwinski , Chaoying Zhang , Derrick Chu , Claire Sampankanpanich , 2 4 5 2 2 Nichole A. Reyes , Abbey Vangeloff , Fangfang Xing , Xudong Li , Ting-Ting Wu , Sudhir 6 1,7 4 2 Sahasrabudhe , Hongyu Deng , Douglas J. LaCount *, Ren Sun * 1 School of Dentistry, University of California Los Angeles, Los Angeles, California, United States of America, 2 Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles, California, United States of America, 3 UCLA DOE-Institute for Genomics and Proteomics, University of California Los Angeles, Los Angeles, California, United States of America, 4 Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West LaFayette, Indiana, United States of America, 5 Department of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America, 6 Prolexys Pharmaceuticals, Salt Lake City, Utah, United States of America, 7 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China Abstract Genome-wide yeast two-hybrid (Y2H) screens were conducted to elucidate the molecular functions of open reading frames (ORFs) encoded by murine c-herpesvirus 68 (MHV-68). A library of 84 MHV-68 genes and gene fragments was generated in a Gateway entry plasmid and transferred to Y2H vec