altered meristem program 1 is involved in development of seed dormancy in arabidopsis改变分生组织程序1参与开发的拟南芥种子休眠.pdfVIP

ALTERED MERISTEM PROGRAM 1 Is involved in Development of Seed Dormancy in Arabidopsis ¤ Jayne Griffiths , Jose M. Barrero, Jennifer Taylor, Chris A. Helliwell, Frank Gubler* Division of Plant Industry, CSIRO, Canberra, Australian Capital Territory, Australia Abstract Mutants in the rice PLASTOCHRON 3 and maize VIVIPAROUS 8 genes have been shown to have reduced dormancy and ABA levels. In this study we used several mutants in the orthologous gene ALTERED MERISTEM PROGRAM 1 (AMP1) to determine its role in seed dormancy in Arabidopsis. Here we report that there are accession-specific effects of mutations in AMP1. In one accession, amp1 mutants produce seeds with higher dormancy, while those in two other accessions produce seeds of lower dormancy. These accession-specific effects of mutating AMP1 were shown to extend to ABA levels. We assayed global gene transcription differences in seeds of wild-type and mutant from two accessions demonstrating opposing phenotypes. The transcript changes observed indicate that the amp1 mutation shifts the seed transcriptome from a dormant into an after-ripened state. Specific changes in gene expression in the mutants give insight into the direct and indirect effects that may be contributing to the opposing dormancy phenotypes observed, and reveal a role for AMP1 in the acquisition and/or maintenance of seed dormancy in Arabidopsis. Citation: Griffiths J, Barrero JM, Taylor J, Helliwell CA, Gubler F (2011) ALTERED MERISTEM PROGRAM 1 Is involved in Development of Seed Dormancy in Arabidopsis. PLoS ONE 6(5): e20408. doi:10.1371/journal.pone.0020408 Editor: Edward Newbigin, University of Melbourne, Australia Received February 22, 2011; Accepted April 19, 2011; Published May 26, 2011 Copyright: 2011 Griffiths et al. This is an open-access article distributed under the terms of the Crea