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mycoplasma suppression of thp-1 cell tlr responses is corrected with antibiotics支原体抑制thp-1细胞tlr反应与抗生素纠正
Mycoplasma Suppression of THP-1 Cell TLR Responses Is Corrected with Antibiotics Ekaterina Zakharova, Jaykumar Grandhi, Mark D. Wewers*, Mikhail A. Gavrilin* Division of Pulmonary Allergy Critical Care and Sleep Medicine, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, Ohio, United States of America Abstract Mycoplasma contamination of cultured cell lines is a serious problem in research, altering cellular response to different stimuli thus compromising experimental results. We found that chronic mycoplasma contamination of THP-1 cells suppresses responses of THP-1 cells to TLR stimuli. For example, E. coli LPS induced IL-1 b was suppressed by 6 fold and IL-8 by 10 fold in mycoplasma positive THP-1 cells. Responses to live F. novicida challenge were suppressed by 50-fold and 40- fold respectively for IL-1b and IL-8. Basal TLR4 expression level in THP-1 cells was decreased by mycoplasma by 2.4-fold (p = 0.0003). Importantly, cell responses to pathogen associated molecular patterns are completely restored by mycoplasma clearance with Plasmocin. Thus, routine screening of cell lines for mycoplasma is important for the maintenance of reliable experimental data and contaminated cell lines can be restored to their baseline function with antibiotic clearance of mycoplasma. Citation: Zakharova E, Grandhi J, Wewers MD, Gavrilin MA (2010) Mycoplasma Suppression of THP-1 Cell TLR Responses Is Corrected with Antibiotics. PLoS ONE 5(3): e9900. doi:10.1371/journal.pone.0009900 ´ ´ Editor: Jerome Nigou, CNRS/Universite de Toulouse, France Received January 14, 2010; Accepted March 5, 2010; Published March 25, 2010 Copyright: 2010 Zakharova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution
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