有哪些信誉好的足球投注网站multiple-locus variable number tandem repeat analysis of staphylococcus aureus comparison with pulsed-field gel electrophoresis and spa-typing金黄色葡萄球菌multiple-locus可变数目串联重复序列分析比较pulsed-field凝胶电泳和spa-typing.pdfVIP
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multiple-locus variable number tandem repeat analysis of staphylococcus aureus comparison with pulsed-field gel electrophoresis and spa-typing金黄色葡萄球菌multiple-locus可变数目串联重复序列分析比较pulsed-field凝胶电泳和spa-typing
Multiple-Locus Variable Number Tandem Repeat Analysis of Staphylococcus Aureus: Comparison with Pulsed-Field Gel Electrophoresis and spa-Typing Leo M. Schouls*, Emile C. Spalburg, Martijn van Luit, Xander W. Huijsdens, Gerlinde N. Pluister, Marga G. van Santen-Verheuvel, Han G. J. van der Heide, Hajo Grundmann, Max E. O. C. Heck, Albert J. de Neeling Laboratory for Infectious Diseases and Perinatal screening, National Institute for Public Health and the Environment, Bilthoven, the Netherlands Abstract Background: Molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) is required to study the routes and rates of transmission of this pathogen. Currently available typing techniques are either resource-intensive or have limited discriminatory ability. Multiple-locus variable number tandem repeat analysis (MLVA) may provide an alternative high throughput molecular typing tool with high epidemiological resolution. Methodology/Principal Findings: A new MLVA scheme for S. aureus was validated using 1681 S. aureus isolates collected from Dutch patients and 100 isolates from pigs. MLVA using 8 tandem repeat loci was performed in 2 multiplex PCRs and the fluorescently labeled PCR products were accurately sized on an automated DNA sequencer. The assessed number of repeats was used to create MLVA profiles consisting of strings of 8 integers that were used for categorical clustering. MLVA yielded 511 types that clustered into 11 distinct MLVA complexes which appeared to coincide with MLST clonal complexes. MLVA was at least as discriminatory as PFGE and twice as discriminatory as spa-sequence typing. There was considerable congruence between MLVA, spa-sequence typing and PFGE, at the MLVA complex level with group separation values of 95.1% and 89.2%. MLVA could not discriminate between pig-related MRSA strains isolated from
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