multiplex detection of b-type natriuretic peptide, cardiac troponin i and c-reactive protein with photonic suspension arrayb型利钠肽的多路检测心肌肌钙蛋白i和c反应蛋白与光子悬浮阵列.pdfVIP

Multiplex Detection of B-Type Natriuretic Peptide, Cardiac Troponin I and C-Reactive Protein with Photonic Suspension Array 1,2. 2. 2 2 2 1 3 Wenbin Lu , Cong Fu , Yong Chen , Jun Lu , Yuyu Yao , Chengxing Shen *, Zhongze Gu * 1 Department of Cardiology, Xinhua Hospital affiliated to Shanghai Jiao tong University, Shanghai, China, 2 Department of Cardiology, ZhongDa hospital affiliated to Southeast University, Nanjing, Jiangsu, China, 3 State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China Abstract A novel photonic suspension array has been developed for multiplex immunoassay. The carriers of this array were silica colloidal crystal beads (SCCBs). The codes of these carriers have characteristic reflection peaks originating from their structural periodicity; therefore they do not suffer from fading, bleaching, quenching or chemical instability. In addition, the fluorescence background of SCCBs is negligible because no fluorescence materials or dyes are involved. With a sandwich method, the proposed suspension array was used for simultaneous multiplex detection of heart failure (HF) and coronary heart disease (CAD) biomarkers in one test tube. The results showed that the three biomarkers: cardiac troponin I (cTnI), C- reactive protein (CRP) and B-type natriuretic peptide (BNP) could be assayed in the ranges of 0.1–500 ng/ml, 1–500 mg/L and 0.02–50 ng/ml with detection limits of 0.01 ng/ml, 0.36 mg/L and 0.004 ng/ml at 3s, respectively. There were no significant differences between the photonic suspension array and traditional parallel single-analyte test. This novel method demonstrated acceptable a