multiplexed dna sequence capture of mitochondrial genomes using pcr products多路复用的线粒体基因组的dna序列捕获使用pcr产品.pdfVIP

Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products ¨ ¨ Tomislav Maricic*, Mark Whitten, Svante Paabo Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany Abstract Background: To utilize the power of high-throughput sequencers, target enrichment methods have been developed. The majority of these require reagents and equipment that are only available from commercial vendors and are not suitable for the targets that are a few kilobases in length. Methodology/Principal Findings: We describe a novel and economical method in which custom made long-range PCR products are used to capture complete human mitochondrial genomes from complex DNA mixtures. We use the method to capture 46 complete mitochondrial genomes in parallel and we sequence them on a single lane of an Illumina GAII instrument. Conclusions/Significance: This method is economical and simple and particularly suitable for targets that can be amplified by PCR and do not contain highly repetitive sequences such as mtDNA. It has applications in population genetics and forensics, as well as studies of ancient DNA. ¨ ¨ Citation: Maricic T, Whitten M, Paabo S (2010) Multiplexed DNA Sequence Capture of Mitochondrial Genomes Using PCR Products. PLoS ONE 5(11): e14004. doi:10.1371/journal.pone.0014004 Editor: Robert C. Fleischer, Smithsonian Institution National Zoological Park, United States of America Received May 31, 2010; Accepted October 26, 2010; Published November 16, 2010 Copyright: 2010 Maricic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction