identification of anaplasma marginale type iv secretion system effector proteins识别红孢子虫属边际iv型分泌系统效应的蛋白质.pdfVIP

Identification of Anaplasma marginale Type IV Secretion System Effector Proteins 1. 2. 3 4 3 Svetlana Lockwood , Daniel E. Voth , Kelly A. Brayton , Paul A. Beare , Wendy C. Brown , Robert A. Heinzen4, Shira L. Broschat1,3* 1 School of Electrical Engineering and Computer Science, Washington State University, Pullman, Washington, United States of America, 2 Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America, 3 Department of Veterinary Microbiology and Pathology and Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, United States of America, 4 Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America Abstract Background: Anaplasma marginale, an obligate intracellular alphaproteobacterium in the order Rickettsiales, is a tick-borne pathogen and the leading cause of anaplasmosis in cattle worldwide. Complete genome sequencing of A. marginale revealed that it has a type IV secretion system (T4SS). The T4SS is one of seven known types of secretion systems utilized by bacteria, with the type III and IV secretion systems particularly prevalent among pathogenic Gram-negative bacteria. The T4SS is predicted to play an important role in the invasion and pathogenesis of A. marginale by translocating effector proteins across its membrane into eukaryotic target cells. However, T4SS effector proteins have not been identified and tested in the laboratory until now. Results: By combining computational methods wit