identification of an endogenous ligand bound to a native orphan nuclear receptor内源性配体的识别绑定到本地孤儿核受体.pdfVIP

Identification of an Endogenous Ligand Bound to a Native Orphan Nuclear Receptor 1.¤ 2. 1 3 1 4 Xiaohui Yuan , Tuong Chi Ta , Min Lin , Jane R. Evans , Yinchen Dong , Eugene Bolotin , 5 1 3 Mark A. Sherman , Barry M. Forman * , Frances M. Sladek * 1 Department of Gene Regulation and Drug Discovery, Gonda Diabetes Research Center, The Beckman Research Institute at the City of Hope National Medical Center, Duarte, California, United States of America, 2 Cell, Molecular and Developmental Biology Graduate Program, University of California Riverside, Riverside, California, United States of America, 3 Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, California, United States of America, 4 Genetics, Genomics and Bioinformatics Graduate Program, University of California Riverside, Riverside, California, United States of America, 5 Department of Biomedical Informatics, The Beckman Research Institute at the City of Hope National Medical Center, Duarte, California, United States of America Abstract Orphan nuclear receptors have been instrumental in identifying novel signaling pathways and therapeutic targets. However, identification of ligands for these receptors has often been based on random compound screens or other biased approaches. As a result, it remains unclear in many cases if the reported ligands are the true endogenous ligands, – i.e., the ligand that is bound to the receptor in an unperturbed in vivo setting. Technical limitations have limited our ability to identify ligands based on this rigorous definition. The orphan rec