有哪些信誉好的足球投注网站hybrid sequencing approach applied to human fecal metagenomic clone libraries revealed clones with potential biotechnological applications杂交测序方法应用于人类的宏基因组克隆库显示克隆与潜在的生物技术的应用程序.pdfVIP
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hybrid sequencing approach applied to human fecal metagenomic clone libraries revealed clones with potential biotechnological applications杂交测序方法应用于人类的宏基因组克隆库显示克隆与潜在的生物技术的应用程序
Hybrid Sequencing Approach Applied to Human Fecal Metagenomic Clone Libraries Revealed Clones with Potential Biotechnological Applications ´ ˇ ´ 1,2 1,2 ´ 1 ´ 1,2 Maria Dzunkova , Giuseppe D’Auria *, David Perez-Villarroya , Andres Moya 1 Joint Unit of Research in Genomics and Health, Centre for Public Health Research (CSISP) - Cavanilles Institute for Biodiversity and Evolutionary Biology, University of ´ ´ Valencia, Valencia, Spain, 2 CIBER en Epidemiologıa y Salud Publica (CIBEResp), Madrid, Spain Abstract Natural environments represent an incredible source of microbial genetic diversity. Discovery of novel biomolecules involves biotechnological methods that often require the design and implementation of biochemical assays to screen clone libraries. However, when an assay is applied to thousands of clones, one may eventually end up with very few positive clones which, in most of the cases, have to be ‘‘domesticated’’ for downstream characterization and application, and this makes screening both laborious and expensive. The negative clones, which are not considered by the selected assay, may also have biotechnological potential; however, unfortunately they would remain unexplored. Knowledge of the clone sequences provides important clues about potential biotechnological application of the clones in the library; however, the sequencing of clones one-by-one would be very time-consuming and expensive. In this study, we characterized the first metagenomic clone library from the feces of a healthy human volunteer, using a method based on 454 pyrosequencing coupled with a clone-by-clone Sanger end-sequencing. Inst
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