有哪些信誉好的足球投注网站heterologous ferredoxin reductase and flavodoxin protect cos-7 cells from oxidative stress不等的铁氧还蛋白还原酶和flavodoxin保护cos-7细胞氧化应激.pdfVIP
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heterologous ferredoxin reductase and flavodoxin protect cos-7 cells from oxidative stress不等的铁氧还蛋白还原酶和flavodoxin保护cos-7细胞氧化应激
Heterologous Ferredoxin Reductase and Flavodoxin Protect Cos-7 Cells from Oxidative Stress ´ 1 1 1,2 3 Marıa G. Mediavilla *, Gisela A. Di Venanzio , Edgardo E. Guibert , Claudio Tiribelli ´ ´ ´ ´ 1 Centro Binacional (Argentina-Italia) de Investigaciones en Criobiologıa Clınica y Aplicada (CAIC) and Consejo Nacional de Investigaciones Cientıficas y Tecnicas ´ ´ ´ ´ (CONICET), Rosario, Argentina, 2 Area Biologıa Molecular, Facultad de Ciencias Bioquımicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Argentina, 3 Centro Studi Fegato, AREA Science Park, Basovizza, and Department ACADEM, University of Trieste, Trieste, Italy Abstract Background: Ferredoxin-NADP(H) reductase (FNR) from Pisum sativum and Flavodoxin (Fld) from Anabaena PCC 7119 have been reported to protect a variety of cells and organisms from oxidative insults. In this work, these two proteins were expressed in mitochondria of Cos-7 cells and tested for their efficacy to protect these cells from oxidative stress in vitro. Principal Findings: Cos-7/pFNR and Cos-7/pFld cell lines expressing FNR and Fld, respectively, showed a significantly higher resistance to 24 h exposure to 300–600 mM hydrogen peroxide measured by LDH retention, MTT reduction, malondialdehyde (MDA) levels and lipid peroxide (LPO; FOX assay) levels. However, FNR and Fld did not e
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