genome-wide chromatin remodeling identified at gc-rich long nucleosome-free regions全基因组染色质重塑gc-rich长nucleosome-free区域的识别.pdfVIP

Genome-Wide Chromatin Remodeling Identified at GC- Rich Long Nucleosome-Free Regions . . Karin Schwarzbauer , Ulrich Bodenhofer , Sepp Hochreiter* Institute of Bioinformatics, Johannes Kepler University, Linz, Austria Abstract To gain deeper insights into principles of cell biology, it is essential to understand how cells reorganize their genomes by chromatin remodeling. We analyzed chromatin remodeling on next generation sequencing data from resting and activated T cells to determine a whole-genome chromatin remodeling landscape. We consider chromatin remodeling in terms of nucleosome repositioning which can be observed most robustly in long nucleosome-free regions (LNFRs) that are occupied by nucleosomes in another cell state. We found that LNFR sequences are either AT-rich or GC-rich, where nucleosome repositioning was observed much more prominently in GC-rich LNFRs — a considerable proportion of them outside promoter regions. Using support vector machines with string kernels, we identified a GC-rich DNA sequence pattern indicating loci of nucleosome repositioning in resting T cells. This pattern appears to be also typical for CpG islands. We found out that nucleosome repositioning in GC-rich LNFRs is indeed associated with CpG islands and with binding sites of the CpG-island-binding ZF-CXXC proteins KDM2A and CFP1. That this association occurs prominently inside and also prominently outside of promoter regions hints at a mechanism governing nucleosome repositioning that acts on a whole- genome scale. Citation: Schwarzbauer K, Bodenhofer U, Hochreiter S (2012) Genome-Wide Chromatin Remodeling Identified at GC-Rich Long Nucleosome-Free Regions. PLoS ONE 7(11): e47924. doi:10.1371/journal.pone.0047924 Editor: Mo