gene expression profiling during conidiation in the rice blast pathogen magnaporthe oryzae基因表达分析在稻瘟病病菌分生孢子生产中magnaporthe oryzae.pdfVIP

Gene Expression Profiling during Conidiation in the Rice Blast Pathogen Magnaporthe oryzae 1 2 Kyoung Su Kim , Yong-Hwan Lee * 1 Department of Applied Biology, College of Agriculture and Life Sciences, Research Institute for Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea, 2 Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Center for Fungal Pathogenesis, Center for Agricultural Biomaterials, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea Abstract Conidiation of phytopathogenic fungi is a key developmental process that plays a central role in their life cycles and in epidemics. However, there is little information on conidiation-induced molecular changes in the rice blast fungus Magnaporthe oryzae. As a first step to understand conidiogenesis in this fungus, we measured genome-wide gene expression profiles during conidiation using a whole genome oligonucleotide microarray. At a two-fold expression difference, approximately 4.42% and 4.08% of genes were upregulated and downregulated, respectively, during conidiation. The differentially expressed genes were functionally categorized by gene ontology (GO) term analysis, which demonstrated that the gene set encoded proteins that function in metabolism, cell wall biosynthesis, transcription, and molecule transport. To define the events of the complicated process of conidiogenesis, another set of microarray experiments was performed using a deletion mutant for MoHOX2, a stage-specific transcriptional regulator essen