gene expression signature in peripheral blood detects thoracic aortic aneurysm在外周血基因表达特征检测胸主动脉瘤.pdfVIP
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gene expression signature in peripheral blood detects thoracic aortic aneurysm在外周血基因表达特征检测胸主动脉瘤
Gene Expression Signature in Peripheral Blood Detects Thoracic Aortic Aneurysm 1. 1. 2 3¤ 2 4 4 Yulei Wang , Catalin C. Barbacioru , Dov Shiffman , Sriram Balasubramanian , Olga Iakoubova , Maryann Tranquilli , Gonzalo Albornoz , 1 1 3¤ 1 1 1 4 Julie Blake , Necip N. Mehmet , Dewi Ngadimo , Karen Poulter , Frances Chan , Raymond R. Samaha *, John A. Elefteriades * 1 Applied Biosystems, Foster City, California, United States of America, 2 Celera, Alameda, California, United States of America, 3 Celera Genomics, South San Francisco, California, United States of America, 4 Section of Cardiothoracic Surgery, Yale University School of Medicine, New Haven, Connecticut, United States of America Background. Thoracic aortic aneurysm (TAA) is usually asymptomatic and associated with high mortality. Adverse clinical outcome of TAA is preventable by elective surgical repair; however, identifying at-risk individuals is difficult. We hypothesized that gene expression patterns in peripheral blood cells may correlate with TAA disease status. Our goal was to identify a distinct gene expression signature in peripheral blood that may identify individuals at risk for TAA. Methods and Findings. Whole genome gene expression profiles from 94 peripheral blood samples (collected from 58 individuals with TAA and 36 controls) were analyzed. Significance Analysis of Microarray (SAM) identified potential signature genes characterizing TAA vs. normal, ascending vs. descending TAA, and sporadic vs. familial TAA. Using a training set containing 36 TAA patients and 25 controls, a 41-gene classificatio
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