foxp3 expression is upregulated in cd4+t cells in progressive hiv-1 infection and is a marker of disease severityfoxp3表达调节cd4 + t细胞在进步的hiv - 1感染和疾病严重程度的一个标志.pdfVIP

+ FOXP3 Expression Is Upregulated in CD4 T Cells in Progressive HIV-1 Infection and Is a Marker of Disease Severity 1,2,4 1,4 1,3,4 4 Melinda S. Suchard *, Elizabeth Mayne , Victoria A. Green , Sharon Shalekoff , Samantha L. 4 1 4 4 Donninger , Wendy S. Stevens , Clive M. Gray , Caroline T. Tiemessen 1 Haematology and Molecular Medicine, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa, 2 Microbiology, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa, 3 Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom, 4 AIDS Virus Research Unit, National Institute for Communicable Disease, Johannesburg, South Africa Abstract Background: Understanding the role of different classes of T cells during HIV infection is critical to determining which responses correlate with protective immunity. To date, it is unclear whether alterations in regulatory T cell (Treg) function are contributory to progression of HIV infection. Methodology: FOXP3 expression was measured by both qRT-PCR and by flow cytometry in HIV-infected individuals and uninfected controls together with expression of CD25, GITR and CTLA-4. Cultured peripheral blood mononuclear cells were stimulated with anti-CD3 and cell proliferation was assessed by CFSE dilution. + + Principal Findings: HIV infected individuals had signif