gene expression responses linked to reproduction effect concentrations (ec10,20,50,90) of dimethoate, atrazine and carbendazim, in enchytraeus albidus基因表达的反应与生殖效应浓度(ec10 20、50、90)乐果、莠去津和多菌灵enchytraeus albidus.pdfVIP

Gene Expression Responses Linked to Reproduction Effect Concentrations (EC10,20,50,90 ) of Dimethoate, Atrazine and Carbendazim, in Enchytraeus albidus 1 2 ´ 1 Sara C. Novais *, Wim De Coen , Monica J. B. Amorim 1 Department of Biology CESAM, University of Aveiro, Aveiro, Portugal, 2 Department of Biology - E.B.T., University of Antwerp, Antwerp, Belgium Abstract Background: Molecular mechanisms of response to pesticides are scarce and information on such responses from soil invertebrates is almost inexistent. Enchytraeus albidus (Oligochaeta) is a standard soil ecotoxicology model species for which effects of many pesticides are known on survival, reproduction and avoidance behaviour. With the recent microarray development additional information can be retrieved on the molecular effects. Methodology/Principal Findings: Experiments were performed to investigate the transcription responses of E. albidus when exposed to three pesticides – dimethoate (insecticide), atrazine (herbicide) and carbendazim (fungicide) – in a range of concentrations that inhibited reproduction by 10%, 20%, 50% and 90% (EC10, EC20, EC50 and EC90, respectively). The goal of this study was to further identify key biological processes affected by each compound and if dose-related. All three pesticides significantly affected biological processes like translation, regulation of the cell cycle or general response to stress. Intracellular signalling and microtubule-based movement were affected by dimethoate and carbendazim whereas atrazine affected lipid and steroid metabolism (also by dimethoate) or carbohydrate metabolism (also by carbendazim). Response to DNA damage/DNA repair was exclusively affected by carbendazim. Conclusion