a large expansion of the hsfy gene family in cattle shows dispersion across yq and testis-specific expression的大规模扩张hsfy基因家族在yq牛显示色散和testis-specific表达式.pdfVIP

A Large Expansion of the HSFY Gene Family in Cattle Shows Dispersion across Yq and Testis-Specific Expression ¤ Christine K. Hamilton, Tamas Revay , Robin Domander, Laura A. Favetta, W. Allan King* Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada Abstract Heat shock transcription factor, Y-linked (HSFY) is a member of the heat shock transcriptional factor (HSF) family that is found in multiple copies on the Y chromosome and conserved in a number of species. Its function still remains unknown but in humans it is thought to play a role in spermatogenesis. Through real time polymerase chain reaction (PCR) analyses we determined that the HSFY family is largely expanded in cattle (,70 copies) compared with human (2 functional copies, 4 HSFY-similar copies). Unexpectedly, we found that it does not vary among individual bulls as a copy number variant (CNV). Using fluorescence in situ hybridization (FISH) we found that the copies are dispersed along the long arm of the Y chromosome (Yq). HSFY expression in cattle appears restricted to the testis and its mRNA correlates positively with mRNA markers of spermatogonial and spermatocyte cells (UCHL1 and TRPC2, respectively) which suggests that HSFY is expressed (at least in part) in early germ cells. Citation: Hamilton CK, Revay T, Domander R, Favetta LA, King WA (2011) A Large Expansion of the HSFY Gene Family in Cattle Shows Dispersion across Yq and Testis-Specific Expression. PLoS ONE 6(3): e17790. doi:10.1371/journal.pone.0017790 Editor: Laszlo Orban, Temasek Life Sciences Laboratory, Singapore Received October 30, 2010; Accepted February 10, 2011; Published March 7, 2011 Copyright: 2011 Hamilton et al. This is an open-access article distributed under the terms of the Creative Commons Attributio