a large gene network in immature erythroid cells is controlled by the myeloid and b cell transcriptional regulator pu.1大基因网络不成熟红细胞细胞由骨髓控制和b细胞转录监管机构pu.1.pdfVIP

A Large Gene Network in Immature Erythroid Cells Is Controlled by the Myeloid and B Cell Transcriptional Regulator PU.1 1. 2. 1 3,4 3 5 Sandeep N. Wontakal , Xingyi Guo , Britta Will , Minyi Shi , Debasish Raha , Milind C. Mahajan , 5 3,4 1 2,6 1 Sherman Weissman , Michael Snyder , Ulrich Steidl , Deyou Zheng *, Arthur I. Skoultchi * 1 Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, United States of America, 2 The Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, New York, United States of America, 3 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, United States of America, 4 Department of Genetics, Stanford University, Stanford, California, United States of America, 5 Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America, 6 Departments of Genetics and Neuroscience, Albert Einstein College of Medicine, Bronx, New York, United States of America Abstract PU.1 is a hematopoietic transcription factor that is required for the development of myeloid and B cells. PU.1 is also expressed in erythroid progenitors, where it blocks erythroid differentiation by binding to and inhibiting the main erythroid promoting factor, GATA-1. However, other mechanisms by which PU.1 affects the fate of erythroid progenitors have not been thoroughly explored. Here, we used ChIP-Seq analysis for PU.1 and gene expression profiling in erythroid cells