a careful look at binding site reorganization in the even-skipped enhancers of drosophila and sepsids仔细看看结合位点重组在果蝇和sepsids甚至没有增强剂.pdfVIP

Perspective A Careful Look at Binding Site Reorganization in the even-skipped Enhancers of Drosophila and Sepsids 1 1 1,2 Emily E. Hare , Brant K. Peterson , Michael B. Eisen * 1 Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, United States of America, 2 Howard Hughes Medical Institute, University of California Berkeley, Berkeley, California, United States of America In our recent PLoS Genetics paper [1] on lines wherever BLAST finds a short stretch represents the degree of similarity. Such the organization and activity of enhancers of sequence similarity (forward- and re- plots for all of the enhancers discussed in our in the even-skipped gene of sepsid flies, we verse-strand hits are distinguished by the paper [1] place the collinearity highlighted described, illustrated, analyzed, and dis- direction of the line). The name ‘‘homol- by Crocker and Erives [2] in context cussed a series of small sequence blocks ogy plot’’ is a misnomer, as most of the hits (Figure 1 and Figure 2) (additional maps conserved between sepsid and Drosophila they display have BLAST scores too low to using different similarity detection methods enhancers. We are thus rather surprised confirm the evolutionary relationship im- and different cutoffs are shown in Figure S2). that Crocker and Erives [2] have an- plied by the term homology—so we will First, the colinear blocks span a region that is nounced the ‘‘discovery’’ of these con- use the more appropriate term ‘‘two- less than half of the length of t