咖啡因增强UVB辐射诱导HaCaT细胞凋亡探究.docVIP

咖啡因增强UVB辐射诱导HaCaT细胞凋亡探究[摘要]目的：观察咖啡因对中波紫外线（Ultraviolet B ，UVB）辐射诱导的HaCaT细胞凋亡的影响。方法：在DMEM 中培养HaCaT细胞，将细胞分成空白对照组（A组），1mM咖啡因处理组（B组），5mM咖啡因处理组（C组），UVB（20mJ/cm2）辐射组（D组），UVB辐射（20mJ/cm2）+1mM咖啡因处理组（E组），UVB辐射（20mJ/cm2）+5mM咖啡因处理组（F组），Annexin V/PI观察各组细胞凋亡比例，Western blot检测各组细胞p21和BCL-2表达情况。结果：20mJ/cm2UVB能诱导HaCaT细胞凋亡增加（A组 凋亡率5.43%，D组凋亡率20.67%），而1mM和5mM咖啡因能进一步促进HaCaT细胞凋亡（E组凋亡率22.25%， F组凋亡率36.61%），UVB 抑制细胞p21和BCL-2蛋白表达，咖啡因能进一步抑制21和BCL-2蛋白表达。结论：UVB辐射能诱导HaCaT细胞凋亡，抑制p21和BCL-2蛋白表达，咖啡因则能进一步促进凋亡，抑制p21和BCL-2蛋白表达。 [关键词]咖啡因；中波紫外线；细胞凋亡 [中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455（2011）07-1105-03 The effect of caffeine to UVB irradiation induced cell apoptosis of HaCaT cells LEI Xia1,LIU Bo2,HE Yu-ying3,WU Jin-jin1,CHENG Qiong-hui1 （1.Department of Dermatology,The Third Affiliated Hospital,The Third Military Medical University,Chongqing 400042,China；2.Department of Orthopaedics,The First Affiliated Hospital,Chongqing Medical University,Chonqing 400036,China；3.Department of Dermatology,University of Chicago,60637,USA） Abstract:ObjectiveTo investigate the effect of caffeine on UVB irradiation induced cell apoptosis of HaCaT cells.MethodsHaCaT cells were cultured in DMEM medium. Cells were divided into 6 groups. A group: Blank control (without UVB and caffeine); B group:1mM caffeine, without UVB; C group: 5mM caffeine, without UVB; D group: without caffeine ,20mJ/cm2 UVB; E group: 1mM caffeine, 20mJ/cm2 UVB ; F group: 5mM caffeine, 20mJ/cm2 UVB. Annexin V/PIKit was used to detect cell apoptosis. Western blot was used to detect theexpression of p21 and BCL-2. Results 20mJ/cm2 UVB irradiation could induce cell apoptosis of HaCaT cells (cell apoptosis rate is 5.43% in group A and 20.67% in group D), 1mM and 5mM caffeine could promote cell apoptosis of HaCaT cells further (cell apoptosis rate is 22.25% in group E and 36.61% in group F). UVB irradiation could inhibite the expression of p21 and BCL-2,caffeine could inhibite p21 and BCL-2 futher.ConclusionsUVB irradiation could induce HaCaT