保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠肝组织病理形态学影响.docVIP

保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠肝组织病理形态学影响摘 要：目的：观察保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠肝组织病理形态学的影响。方法：60只小鼠随机分为6组，分别以相应剂量药物灌胃5天，再以雷公藤多苷灌胃造模，制备病理切片，光镜下观察，并对肝细胞变性情况进行半定量分析。结果：模型组肝细胞出现不同程度变性和凋亡细胞等病理改变，保肝解毒颗粒大、中剂量组脂肪变性较轻、肝窦稍有变窄。半定量分析结果表明，模型组肝细胞变性程度严重，与空白组比较有统计学差异（P0.05）。结论：保肝解毒颗粒具有良好的保肝作用。 ? 关键词：保肝解毒颗粒；药物性肝损伤；雷公藤多苷；病理形态学研究；半定量分析? 中图分类号：R285.5 文献标识码：A文章编号： 1673-7717（2008）11-2406-03 ?? Effects of Baogan Jiedu Granule on Pathomorphology of Hepatic Tissues in ?Acute Liver Injury Mice Induced by Tripterygium Glycosides ?? LU Bao?ping，PENG Bo，LI Jian?guo?（Institute of Hepatic Disease，He’nan University of TCM，Zhengzhou 450008，Henan，China）?? Abstract：Objective：To explore the effects ofBaogan Jiedu Granule on pathomorphology of hepatic tissues in acute liver injury mice induced by tripterygium glycosides. Methods：60 mice were randomly divided into tripterygium glycosides model group，big-dosage，medium-dosage and small-dosage group ofBaogan Jiedu Granule，Ganlixin group and control group. Each group was respectively perfused with corresponding drug for 5 days and tripterygium glycosides was perfused once to make the model. Pathological section of hepatic tissues were prepared and observed under light microscope and the conditions of liver cell degeneration were semi-quantitatively analyzed. Results：Hepatocyteof the model group appeared different-degree degeneration and apoptosis. Fatty degeneration of the big-dosage and medium-dosage of Baogan Jiedu Grannule was light，and sinus hepaticus became a little narrow. Results of semi-quantitative analysis indicated that the liver cell degeneration degree of model group was statistically significantly severer than that of the control（P0.05）. Conclusion：Baogan Jiedu Granule has a good hepatoprotective effect for liver injury. ? Key words：Baogan Jiedu Granule；drug-induced liver injury；tripterygium glycosides；pathomorphology study；semi-quantitative analysis ?? 保肝解毒颗粒由芦荟、刺蒺藜、大枣等药物组成，具有清热燥湿、凉肝解毒、疏肝理气、健脾和胃之功效，是课题组在多年实践中总结出来的防治药物性肝损