人脐带间充质干细胞及蚕丝蛋白支架构建组织工程脂肪探究.docVIP

人脐带间充质干细胞及蚕丝蛋白支架构建组织工程脂肪探究[摘要]目的：将体外以人脐带间充质干细胞（hUCMSCs）与蚕丝蛋白支架初步构建的组织工程脂肪移植到大鼠体内，观察其演变过程。 方法：hUCMSCs与蚕丝蛋白支架复合培养10天后，进行成脂诱导；6周后将其移植到Wistar大鼠后肢肌肉内，同时，以同体积支架材料作为对照；分别于移植后4周和8周取材，行油红O染色、HE染色以及扫描电镜观察。结果：hUCMSCs与蚕丝蛋白支架复合培养及成脂诱导6周后，见大量成脂样细胞生成，并与支架牢固粘附。移植4周，移植物体积略小，质稍硬，表面有透明薄膜形成，膜中分布新生血管网；油红O染色见支架内新生脂肪组织及细胞呈橙红色；HE染色显示支架网眼内有新生脂肪组织，并可见少量炎性细胞浸润；扫描电镜见支架网眼内有球形、表面光滑的脂肪细胞。移植8周，移植物体积进一步缩小，质变软，表面薄膜内血管网丰富；油红O染色见支架中着橙红色组织较前明显增多，部分呈片状融合；HE染色显示新生脂肪明显增多，仍有少量炎性细胞浸润；扫描电镜显示脂肪细胞较前增生明显。对照组同样可见炎性细胞浸润，未见新生脂肪组织生成，支架材料8周时较4周时降解更加明显。结论：随着时间推移，蚕丝蛋白支架网眼内脂肪细胞逐渐增多，支架材料在体内呈现逐步降解趋势，说明体内环境有利于组织工程化脂肪的进一步形成。同时，也提示支架材料在组织相容性方面尚存不足。 [关键词]人脐带间充质干细胞；蚕丝蛋白；支架材料；脂肪组织；组织工程学 [中图分类号]R318 [文献标识码]A [文章编号]1008-6455（2011）05-0774-03 Study on reconstruction of tissue engineering adipose with human umbilical cord mesenchymal stem cells and silk fibroin scaffolds LIU Yi, XIAO Hong-tao （PLA Center of Burns and Plastic Surgery, Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, Gansu, China） Abstract:ObjectiveTo transplant the tissue engineering adipose reconstructed with human umbilical cord mesenchymal stem cells (hUCMSCs)and silk fibroin scaffolds in vitro into Wistar rats, and dynamically observe their changing.MethodshUCMSCs and silk fibroin scaffolds were compoundly cultured ten days, theninduced them into adipose and continuely cultured. Six weeks later, those compound materials were transplanted into muscles in hind legs of Wistar rats, at the same time, the pure scaffolds in same volume were transplanted as control. The samples were harvested at 4 and 8 weeks respectively. Oil Red-O stain, H.E. stain and scanning electron microscope were employed to observe their changes.ResultsA lot of adipose-like cells appearred and firmly adhered to scaffolds six weeks later after hUCMSCs and silk fibroin scaffolds were compoundly cultured and induced into adipose. Four weeks later after transplantation, the volume of transplant became slightly smaller and the quality of it was a little hard. There was