大肠癌parg与parp关系的初步研究 - 第三军医大学学报.docVIP

大肠癌中PARG对PARP的调节作用及意义 李 佳，林 玲，王娅兰（重庆医科大学基础医学院病理学教研室，重庆400016） 摘 要：目的 初步探讨大肠癌中聚(腺苷二磷酸核糖)水解酶[Poly(ADP-ribose) glycohydrolase, PARG]与聚(腺苷二磷酸核糖)聚合酶[Poly(ADP-ribose) polymerase, PARP]的关系。方法 应用S-P免疫组化检测人大肠癌组织中PARG、PARP的表达；以PARG抑制剂丹宁酸（Gallotannin，GLTN）为处理因素，采用流式细胞计数检测小鼠结肠腺癌CT26细胞中PARG、PARP表达变化。结果 PARG和PARP在人大肠癌组织中的表达呈正相关（r =0.30001，P0.05）；流式细胞计数显示，用GLTN处理后，小鼠结肠腺癌CT26细胞中PARG、PARP的阳性细胞率分别较对照组降低，差异具有统计学意义（P0.05）。结论 大肠癌中PARG和PARP的表达有相关性，PARG抑制剂可以抑制PARG及PARP表达，PARG可能对PARP具有调节作用。 关键词：聚(腺苷二磷酸核糖)水解酶；聚(腺苷二磷酸核糖)聚合酶；大肠癌 中图法分类号：R73 文献标识码：A Regulatory effects of PARG on PARP in colorectal carcinoma LI Jia, LIN Ling, WANG Ya-lan (Department of Pathology, Chongqing Medical University, Chongqing 400016, China) Abstract: Objective To study the relationship between Poly(ADP-ribose) glycohydrolase (PARG) and Poly(ADP-ribose) polymerase (PARP) in colorectal carcinoma. Methods Immunohistochemical S-P staining was used to examine the expressions of PARG and PARP in human colorectal carcinoma specimens. The positive rate of PARG and PARP in murine colon carcinoma CT26 cells was detected by flow cytometry before and after the treatment of PARG inhibitor Gallotannin. Results PARG and PARP expressed in colorectal carcinoma specimens was of positive correlation (r=0.30001, P0.05). The expressions of PARG and PARP were weaker in Gallotannin-treated CT26 cells than those in control cells (P0.05). Conclusion The expressions of PARG and PARP are correlated positively in colorectal carcinoma. PARG inhibitor inhibits the expressions of both PARG and PARP, indicating that PARG may regulate PARP. Key words: Poly(ADP-ribose) glycohydrolase; Poly(ADP-ribose) polymerase; colorectal carcinoma 在细胞内众多蛋白质的聚ADP糖基化过程中，聚(腺苷二磷酸核糖)聚合酶[Poly(ADP-ribose) polymerase, PARP]和聚(腺苷二磷酸核糖)水解酶[Poly(ADP-ribose) glycohydrolase, PARG]发挥着重要的作用[1]。研究表明，PARP与多种疾病的发生、发展有关[2-4]。而在炎症性疾病的聚ADP糖基化过程中，PARG对PARP的活性则起着重要的调节作用，抑制PARP和PARG能在同等程度上降低缺血再灌注引起的肠组织损伤，抑制PARG能有效的抑制PARP活性[5-6]。但在肿瘤中，两者关系如何，尚少见文献报道。 基金项目：重庆市自然科学基金(2006BB52