影响水样菌落总数的多因素研究.pdfVIP

实用预防医学 2008年6月 第15卷 第3期 Practical Preventive Medicine，Jun．2008，Vol 15，No．3 887 文章编号：1006—3110(2008)03—0887—02 影响水样菌落总数的多因素研究 张勇兵 摘要： 目的 探讨影响水样菌落总数结果的主要因素。 方法 观察培养时间(24、48、72 h)；培养温度(35℃、37 ℃、40℃)；样品接种时间(0、20、40、60、90、120 min)；营养琼脂倾注时的温度(45℃、48℃、50℃、55℃)；分别对水样菌落 总数进行测定，并进行统计学分析。 结果 培养24 h与48 h菌落总数结果之间存在显著性差异，培养48 h与72 h菌 落总数结果之间无显著性差异；培养温度37℃时，水样菌落总数检出率略高，不同培养温度之间结果无显著性差异；立即 接种与20 min接种，两者之间结果无显著性差异，40 min后接种均存在显著性差异；45 13与48℃之间结果无显著性差 异，50℃后结果之间均存在显著性差异。 结论 菌落总数的培养时间以48 h为宜；培养温度37℃时检出率略高；样品 接种时间应控制在20 min之内为宜；营养琼脂倾注时的温度应为45℃左右，不能超过50℃。 关键词： 水样；菌落总数；多因素；研究 中图分类号：R123 文献标识码：A Study on Multiple Factors Influencing Total Numbers of Colony in Water Samples ZHANG Yong—bing(Center for Disease Control and Prevention of Yiyang，Yiyang 413000，Hunan) Abstractl- 0hiective To investigate the major factors influencing total numbers of colony in water．samples． Methods Total numbers of colony in water samples were measured at the culture time of 24，48，and 72 hours，culture temperature of 35，37，and 40℃，inoculating duration of 0，20，40，60，90，and 120 Iminutes，nutrient agar pouring temperature of 45，48， 50．and 55℃ respectively． And the statistical arialysis was performed． Results There was a significant difference in total lnumbers of colony between culture duration of 24 and 48 hours，and however no significant difference was found between cul ture duration of 48 and 72 hours．When at the culture temperature of 37℃，the totall numbers of colony were slightly increased， without significant difference among the varying culture temperatures．{No considerable difference was noted in total numbers of colony at the time of between immediate inoculating and inoculating within 20 minutes，but there was a marked difference when compared with that at the inoculatin