托吡酯对戊四氮致癫癎大鼠海马AQP4表达水平的影响.pdfVIP

· 166 · Stroke and Nervous Diseases ， Jun 2008，Vo1．15，No．3 托吡酯对戊四氮致癫痫大鼠海马AQP4 表达水平的影响 但毕堂 许 康 张晓琴 【摘要】 目的 探讨托吡酯对戊四氮致癫痫大鼠海马AQP4表达水平的影响。方法 将3O只Wistar 大鼠随机分为戊四氮致癫痫组、托吡酯干预组和正常对照组，每组各10只；癫痫模型点燃后在不同时相点灌 注取材，通过HE染色观察大鼠海马神经元的变化，并应用免疫组化法检测大鼠海马AQP4表达水平。结果 HE染色显示托吡酯干预组神经元变性和坏死较戊四氮致癫痫组明显减轻；免疫组化显示戊四氮致癫痫组 在致癫痫后12 h AQP4的表达显著增强，致癫痫后24 h达高峰，托吡酯干预组在致癫痫后12 h～36 h各时相 点AQP4表达水平均分别低于戊四氮致癫痫组相应时间点(P()．05)。结论 托吡酯通过下调大鼠海马 AQP4的表达可能参与了对大鼠海马神经元的保护过程。 【关键词】 癫痫 托吡酯 海马 戊四氮 AQP4 【中图分类号】 R742．1 【文献标识码】 A 【文章编号】 1007—0478(2008)03—0166—04 Effect of Topiramate on aquaporin-4 expression of hippocampus in seizures rats induced by PTZ Dan Bi— tang ，XuKang ，ZhangXiaoqin． Department ofNeurology ofXinhua Hospital ofHubei，Wuhan 430015 [Abstract] Objective To investigate the effect of topiramate on AQP4 expression of hippocampus in sei— zure rats induced by pentylenetetrazole(PTZ)．Methods Tirty adult Wistar rats were randomly divided into control(c)group，PTZ group and TPM group after kindling of acute epilepsy，then perfused to acquire the brain at the different point．The hippocampus was studied histologically with HE staining and immunohisto— chemically for the expression of AQP4．Results Neuronal degeneration and necrosis in CA1、CA3 and I)C areas of the hippocampus were more remarkable histologically in the pentylenetrazole—treated group than that in the topiramate plus pentylenetrazole treated group According to HE method．The expression of AQP4 positive cell in hippocampus in PTZ group increased gradually afer 12 hour，then in the peak afer 24 hour，and compared with TPM group，it was significantly different(P0．05)．Conclusions Topiramate has neuroprotective