双波长法测定强力治癫散中卡马西平的含量.pdfVIP

现代中西医结合杂志Modern Journal of Integrated Traditional Chinese and Western Medicine 2008 Jan，17(2) ·175· 双波长法测定强力治癫散中卡马西平的含量 王 雷 ，刘冀清 ，薛庆林 ，韩春超。，刘启萍 (1．山东中医药大学第二附属医院，山东济南250001；2．山东中医药大学，山东济南250014) [摘要] 目的 应用双波长光谱测定强力治癫散中卡马西平的含量。方法 在碱性条件 下，选择波长280 nm为测定波长，270 nm为参比波长，消除中药成分的干扰，对卡马西平在两波 长处有不同的紫外吸收光谱，测得吸收度差值(△A)为定量依据。结果 平均回收率98．54％， RSD为0．86％。结论 双波长紫外分光光度法可以作为强力治癫散中卡马西平的含量测定， 其方法简单、准确。 [关键词】 强力治癫散；卡马西平；双波长紫外分光光度法；含量 [中图分类号] R一33 [文献标识码] A [文章编号] 1008—8849(2008)02— 0175—02 Content determination of Carbamazepine in Qianglizhidian Powder with double wavelength method Wang Lei ，Liu Jiqing ，Xue Qinglin ，Han Chunchao2，Liu Qiping (1．The Second Affiliated Hospital of Shandong Traditional Chinese Medical University，ji’nan 250001，Shandong，China； 2．Shandong Traditional Chinese Medical University，Ji’nan 250014，Shandong，China) Abstract：Objective It is tO apply double wavelength spectrum tO determine the content of Carbamazepine in Qian— glizhidian Powder．Methods Under alkalinity condition，the wavelength 280 nm was chosen as determination wavelength， the wavelength 270 nm was chosen as reference wavelength and the interference of traditional Chinese medicine was eliminat— ed．Carbamazepine had different ultraviolet absorption spectrum at the two wavelengths。and the absorbability difference val— ue(△A)determined was quantitation basis．Results The recovery rate was 98．54％ and RSD was 0．86％．Conclusion Double wavelength ultraviolet spectrophotometry can be used tO determine the content of Carbamazepine in Qianglizhidian Powder，and the method is simple and accurate． Key words：Qianglizhidian Powder；Carbamazepine；double wavelength ultraviolet spectrophotometry；content 强力治癫散是以丹参、郁金、石菖