双青咽喉片中绿原酸与甘草酸的含量测定.pdfVIP

药物分析杂志Chin J Pharm Anal 2007，27(12) ·-——1903．-—— 双青咽喉片中绿原酸与甘草酸的含量测定 何兵，冯文宇，田吉，李春红，艾洪兵 (泸州医学院药学院药物研究所，泸州646o0o) 摘要 目的：建立双青咽喉片中绿原酸和甘草酸含量测定方法。方法：采用高效液相色谱法。色谱柱：Dikma Kromasil c 柱 (250 mm x4．6 lnln，5 m)；绿原酸的测定，流动相为甲醇一0．08 mol·L 磷酸溶液(22：78)，流速：1．0 mL·min～，检测波长： 326 rim；甘草酸的测定，流动相为甲醇一0．2 tool·L 醋酸铵一冰醋酸(66：34：1)；流速：0．8 mL·rain～；检测波长：252 am。 结果：绿原酸在0．08—0．8 g范围内具有良好的线性关系(r=0．9999)，平均加样回收率为98．90％(n=12)；甘草酸的线性范 围0．4～4 g(r=0．9999)，平均加样回收率为99．04％(n=12)；。结论：该方法简便、快速、准确，具有良好的重复性和回收 率，可作为该制剂的定量分析方法。 关键词：HPLC；双青咽喉片；绿原酸；甘草酸；含量测定 中图分类号：R917 文献标识码：A 文章编号：o254—1793(2007)12—1903—03 HPLC determination of chlorogenic acid and glycyrrhizic acid in Shuangqingyanhou tablets‘ Shuan ranh~ tabh；ts HE Bing，FENG Wen—yu，TIAN ji，LI Chun—hong，AI Hong—bing (Institute of Materia Medica of Luzhou MedicM College，Luzhou 646000，China) Abstrcat Objective：To establish an HPLC method for the determination of chlorogenic acid(CHA)and glycyr- rhizic acid(GA)in Shuangqingyanhou tablets．Methods：The separation was carried out on a Dikma Kromasil Cl8 column(250 mm×4．6 mm，5 Ixm)．The determination of CHA with methanoI一0．08 moI·L phosphoric acid solution(22：78)as the mobile phase，the flow rate was 1．0 mL·min～and the detection wavelength was 326 nm； The determination of GA with methanol一0．2 mol·L～ammonium acetate—glacial acetic acid(66：34：1 1 as the mobile phase，the flow rate was 0．8 mL·min～and the detection wavelength was 252 nm．Results：The calibration curves showed good linearities over the range of0．08—0．8 g(r=0．9999)for CHA and 0．4—4 g(r：0．9999) for GA．The average recoveries were 98．90％ with for CHA and 99．04％ for GA．Conclusion：This method is sim— pie，rapid，accurate and with good repeatability and recovery，it call be used as a quantitative analysis method for this preparat