不同倍性何首乌中二苯乙烯苷和蒽醌类的含量比较.pdfVIP

呻蒜材 Journal of Chinese Medicinal Materials 第30卷第12期2007年12月 ．1487． · 栽培与育种· 不同倍性何首乌中二苯乙烯苷和 蒽醌类的含量比较 焦旭雯，张相年，赵 翔，严 萍，赵树进 (广州军区广州总医院药学部，广东广州510010) 摘要 目的：考察二倍体与三倍体何首乌中二苯乙烯苷、蒽醌类成分的含量差异。方法：采用Inertsil ODS．3 C。。柱，分别用乙腈-水(25：75)、甲醇-0，1％磷酸(85：15)作为流动相，在320 nm和254 nm处检测2，3，5，4 ．四羟基 二苯乙烯-2-O·母-D-葡萄糖苷、蒽醌类成分的含量。结果：广东德庆二倍体何首乌中二苯乙烯苷的含量最高，广西靖 西三倍体何首乌中结合型蒽醌的比例达到了85％。结论：不同倍性何首乌中化学成分存在差异。 关键词 何首乌；2，3，5，4-四羟基二苯乙烯-2一O—B．D．葡萄糖苷；蒽醌类 中图分类号：R282，1 文献标识码：A 文章编号：1001 54(2007)12—1487-03 Comparison of Content of 2，3，5，4-Tetrahydroxystilbene-2-O-肛D-Glucoside and Anthraquinon in Different Ploidy Radix Polygoni MIIl衄 ori JIAO Xu—wen，ZHANG Xiang-nian，ZHAO Xuan，YAN Ping，ZHAO Shu-jin (Department of Pharmacy，Guangzhou General Hospital of Guangzhou Military Command，Guangzhou 5 10010，China) Abstract Objective：To investigate the content of 2，3，5，4-tetrahydroxystilbene一2一O一8一D-glucoside and anthraquinon in diploid and triploid Radix Polygoni Muhiflori．Methods：4 batches of Radix Polygoni Muhiflori were collected from different districts．The con- tent of 2，3，5，4-tetrahydroxystilbene-2-O一8·D—glucoside and anthraquinon in these samples were determined at 320nm and 254nm wave length by HPLC with Inertsil ODS-3 Cl8 pillar and acetomitrile：aqua(25：75)，methanol：0．1％ phosphoric(85：15)respectively as the mobile phase．Results：The maximum content of 2，3，5，4-tetrahydroxystilbene·2一O一8一D-glucoside was diploid Radix Polygoni Muhiflori from Deqing Guangdong，The maximum ratio of total anthraquinon was triploid Radix Polygoni Muhiflori from Jinxi Guangxi reached 85％．Conclusion：The content of anthraquinon varies greatly in the sam ples from the different producing areas， Key words Radix Polygoni Muhiflori；2，3，5，4 一tetrahydroxystilbene-2一O-B-D—glucoside；Anthraquinon