热休克蛋白27在正畸牙张力侧牙周组织中的表达.docVIP

热休克蛋白27在正畸牙张力侧牙周组织中的表达 聂 晶1，李泽慧2，姜 涵2，潘 旭1，李春霞1 (新疆医科大学第一附属医院，1口腔正畸科，2牙周粘膜科，新疆维吾尔自治区乌鲁木齐市 830054) 引用本文：聂晶，李泽慧，姜涵，潘旭，李春霞. 热休克蛋白27在正畸牙张力侧牙周组织中的表达[J].中国组织工程研究，2017，21(): 1197-1201. DOI:10.3969/j.issn.2095-4344.2017.08.009 ORCID: 0000-0002-8580-9946(聂晶) 文章快速阅读： 文题释义： 热休克蛋白27(HSP-27)：属于低分子量热休克蛋白家族的成员，Hsp27是一个涉及到药物抗性、细胞生长、细胞凋亡、肿瘤的发生和转移等功能的重要蛋白，Hsp27介导的这些功能可能与其对其它蛋白质的影响有关。细胞在没有受到胁迫时，Hsp27表达量比较低而且主要呈大的聚合体，这种聚合体下的Hsp27一般没有活性。当细胞在受到胁迫时，Hsp27表达量会增加而且会被磷酸化，使Hsp27具有活性，执行其一些功能。蛋白质磷酸化是蛋白质的一种主要修饰方式，Hsp27的磷酸化位点有3个Ser-15、Ser-78、Ser-82，当Hsp27在这3个位点磷酸化后，大的聚合体就会解聚形成小的四聚体。Hsp27磷酸化主要由MAPKAPK2、MAPKAPK4、PKC、PKD以及cGMP依赖的蛋白激酶来催化完成。 正畸：Expression of heat shock protein 27 in the periodontium on the tension side during orthodontic tooth movement Nie Jing1, Li Ze-hui2, Jiang Han2, Pan Xu1, Li Chun-Xia1 (1Department of Orthodontics, 2Department of Periodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China) Abstract BACKGROUND: A large number of cytokines produce in the periodontium on the tension side during orthodontic tooth movement, participate in periodontal remodeling, and make the tooth move to its normal position, thus achieving alveolar remodeling. Heat shock protein 27 (HSP27) is reported to be involved in the periodontal remodeling. OBJECTIVE: To investigate the expression of HSP27 in the rat periodontium on the tension side during the orthodontic tooth movement. METHODS: The rat models of orthodontic tooth movement were constructed using NiTi coil spring with the force value of 50 g. The periodontal samples were collected at 1, 3, 5, 7, 10 and 14 days after exerting force to detect the expression level of HSP27 in the periodontium on the tension side. RESULTS AND CONCLUSION: Immunofluorescence staining results revealed that the expression level of HSP27 in the periodontal tissues began to increase at the 1st day after exerting force, reached the highest level at the 3rd day, and then fell to the init