第五章 基因识别.pptVIP

CpG island 预测 Obs/Exp, Y 值0.6 GC含量大于50% 长度几百至几千bp www.ebi.ac.uk/Tools/emboss/cpgplot/index.html CPGPLOT islands of unusual CG composition EMBOSS_001 from 1 to 7740 Observed/Expected ratio 0.60 Percent C + Percent G 50.00 Length 200 Length 418 (4718..5135) Length 286 (5595..5880) /berry.phtml?topic=polyahgroup=programssubgroup=promoter 3’ UTR 区 LDF- 权重 Promoter 与转录因子结合位点分析工具 Promoter2.0 predicts transcription start sites of vertebrate PolII promoters in DNA sequences. Promoter 2.0 Prediction Server http://www.cbs.dtu.dk/services/Promoter/ 在“Promoter 2.0”网页粘贴D63710序列 分析结果 在Proscan网页粘贴序列（FASTA格式） 分析结果 /molbio/proscan/ 分析举例 PROSCAN http://www.cbrc.jp/research/db/TFSEARCH.html TF2 SEARCH 启动子分析网址: /seq_tools/promoter.html http://alggen.lsi.upc.es/recerca/menu_recerca.html http://www.cbs.dtu.dk/services/Promoter/ /~molb470/ ... s/solorz/index.html /molbio/proscan/ http://bip.weizmann.ac.il/toolbo ... ters.html#databases /seq_tools/promoter.html .sg/promoter/CGrich1_0/CGRICH.htm /pub/programs.html#pmatch .hk/~b400559/arraysoft_pathway.html#Promoter http://www.dna.affrc.go.jp/PLACE/signalup.html http://intra.psb.ugent.be:8080/PlantCARE/ http://www.cbs.dtu.dk/services/Promoter/ /molbio/proscan/ /molbio/signal/ /thread-41571-1-1.htm 启动子分析网址: 核苷酸数据库中选择DNA序列(AF319968) ，试用不同的分析工具分析其基因结构，并将分析结果与核苷酸数据库中的结果相比较。预测上述序列是否含有启动子区域，分析其转录因子结合位点。 作业（6） * * * * GCAACACCAGGTCCTGCCCTCCCAGAACTCATGCTGTAGTATGGGAGCAGGGGAGGGACAGGGCAGGGCTGGGTGACACCTGGGAGAAGAGTCCAGGCAGAAGGAGCAGGGAAGGTGAGGTCCTTGGGGAGGATATGGCTGGGCAGGGGGGCTCATGGGGCTGAGGGAGGGAGCAGGGGTGTAGAATGTGGGTCTGCTCTAGGGGTGCTGGGAGGCAGCAGAGGGTTTGCATAGGTGTGGGATAATCTGACTTCAAGTGTTAAAGGGACCTCTCTGGCTGCCACATAGAAGAGGATGAATGGGCCCTGGGTAGCAGTGGTGGAGGATGCGAGGGAAGTCAGAATCCAGGCAGACCTGGGATGGCGGGACATGGGAGGGGGAGGGGAAGGTGAGTCAAGAATGACCTGAGGCAGAGCTGGGCATGGGCTCACACCTGCCATGGGCTCACACCTATAATCCTAGTGCTTTGGGAGGCGAAGGCAAGAGGACCACTTGAGGCCAGGAGTTTGAGAAGAGCCTGATTAACACAATGAGACCTCATCTTTACAAAAAAGCAAAAGGAACAACCCATGGCTTTGGCTTAGGCATGACAGGAAGGGACTGGCAGTCTGAT