运动和葡萄籽原花青素对高脂饮食大鼠血脂代谢及自由基水平.PDFVIP

2013年，第28卷，第4期 ·基础研究· 运动和葡萄籽原花青素对高脂饮食大鼠 血脂代谢及自由基水平的影响* 许思毛1 上官若男2 王第亮1 苏全生2 彭峰林1 摘要 目的：探讨长期中等负荷耐力运动和葡萄籽原花青素(GSP)干预对血脂异常及自由基水平异常的预防作用。 方法：32只大鼠随机分为4组：普通饲料组 (A组)、高脂饮食组 (B组)、高脂饮食+GSP组(C组)、高脂饮食+运动组 (D 组)。B、C与D组大鼠采用高脂饮食喂养。C与D组大鼠在高脂饮食喂养的同时，分别给予GSP、中等负荷耐力跑台 运动干预9周；不进行GSP干预的其他三组采用同等体重剂量安慰剂干预。比较各组大鼠血清总胆固醇(TC)、甘油 三酯(TG)、低密度脂蛋白胆固醇（LDL-C）与高密度脂蛋白胆固醇(HDL-C)及超氧化物歧化酶(SOD)、丙二醛(MDA)水 平。 结果：B、C与D组大鼠血清TC、TG、LDL-C浓度明显升高及HDL-C浓度明显下降，血清SOD活性明显下降及MDA 含量明显增加。但C、D组大鼠血清TC、TG、LDL-C浓度均比B组低，其中D组TG、LDL-C水平又比C组低；D组 HDL-C浓度比B组高；C与D组大鼠血清SOD、MDA指标分别高于、低于B组。 结论：长期中等负荷耐力运动和GSP干预均对高脂饮食大鼠血脂异常形成具一定抵抗作用，但GSP效果不如运动； 两者均可减轻血脂异常大鼠SOD活性的下降程度，减少脂质过氧化反应。 关键词 耐力运动；葡萄籽原花青素；血脂异常；自由基；预防 中图分类号：R493 文献标识码：A 文章编号：1001-1242（2013）-04-0325-05 The intervention effects of exercise and grape seed proanthocyanidins on lipid metabolism and free radi? cal level in rats fed with high-fat-diet/XU Simao，SHANGGUAN Ruonan，WANG Diliang，et al.//Chinese Journal of Rehabilitation Medicine，2013，28(4):325—329 Abstract Objective: To explore the preventive effects of long-term medium load endurance exercise and grape seed proan- thocyanidins ( GSP ) intervention on abnormal levels of lipid and free radicals. Method:A total of 32 rats were randomly divided into 4 groups: normal diet group ( roup A), high fat diet group ( group B), high fat diet plus GSP group (group C), high fat diet plus exercise group (group D).Group B, C and D rats were fed with high-fat diet. At the same time groups C and D rats with high-fat diet feeding were respec- tively given GSP or medium load endurance run intervention for 9 weeks. In other three groups without GSP in- tervention, were intervened with the same weight dose of placebo. Rats serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol(HDL-C) and superoxide dis- mutase(SOD), malondialdehyde (MDA ) levels were compared. Result: In groups B, C and D rats serum TC, TG, LDL-C concentrations increased significantly and HDL-C concentrati