肠道菌群正常参考值的检测.pdfVIP

31 4 中国微生志学杂志 2001年12月第iS卷第6期 Chinese Jou rnal of Microeco【0gy．Decen％ber gO01．V。[13 NoB 艾章编号：1005 376X(2001)06 031 4 02 【论 著】 肠道菌群正常参考值的检测 是仲文．李兰娟，马伟杭，俞云松，陈亚岗 t浙江大学医学院附属一院传染科，浙江杭州 310003) 摘要 目的：定性定量检测并比较抗州地区健康青壮年及老年人群肠道主要菌群(双歧杆菌属、类杆 菌属、消化链球菌属、产气美膜棱菌、乳杆菌属、肠杆菌科、晒球菌属及酵母菌等)的正常参考值，同时计算 并比较双歧杆菌属细菌数量与肠杆菌科细菌数量的对数值比值(B．／E值)。方法 采用光冈法 。结果：老年 组肠道双歧杆菌数量及B／E值较青壮年组均显著减少(P0 03，P0 001)；消化链球苗、晒杆菌科及聒 球菌纽菌数量显著增加(PO．01，P0 001．P0 95)。结论；在获得晒道菌群正常参考值的同时，发现随 着年龄的增加，老年^翰道双歧杆菌数量明显减少．肠杆菌科及晒球菌细菌数量显著增加。 关键词：晒道菌群：参考值 中图舟类号；R378 文献标识码：A INVESTIGATIoN 0F THE NoRMAL HUMAN INTESTINAL MIRCoFLoRA IN HANGZHoU WU Zhong—wen，LI Lan—juan，MA Wei hang，et a1． (Departments 0f lnJ~ctious Diseases，The Ftrn AfJ}liated Hospital，College of Medicine，ZheJiang Universi— ty，Hangzhou 310003，China) Abstract：Objectives：To investigate the intestinal m[croftora[Bifidobcterium，Bacteriodes，Peptostrep— tococcus，Lactobacillus，C．pe ingens，Enterobacteriaceae，E~teroco~~us．Yeasts，and the ratio of Bifidobeteri一 “m to Enterobacteriaceae(B／E ratio)]of healthy volunteers(group 1 whose age arrage from eighteen to sixty，group 2 above sixty)in Hangzhou Method：The method established by Mitsuoka was utd[zed to quantitatively and qualitativety anatyze the feca[flora．Results：The number of fecat Bifido妇cterium，as well the B／E ratio in group 2 were significantty decreased compared with that of group l(尸0．05，，J 0 001)The number of尸 ￡。5f 。c c“ ，Enterobacterlaceae，and Yeasts in group 2，however，was signifi— cant]v increased compared with that of group l(，J 0．0l，尸0．001．尸0 05)Conclasion：The normal va]ues of the fecal f Lora are gained，and the resu]ts above suggest that the numbet of fecalfidobacterium is decreasing with aging，while the numbe r of Peptostreptocmcus，Enterobacteriaceae an