Phylogenetic analysis of the E2 gene of classical swine fever viruses from Lao PDR》.pdfVIP

Virus Research 104 (2004) 87–92 Short communication Phylogenetic analysis of the E2 gene of classical swine fever viruses from Lao PDR Stuart D. Blacksell a,c,∗, Syseng Khounsyb , David B. Boyle a , Irene Greiser-Wilke d , Laurence J. Gleeson a , Harvey A. Westbury a , John S. Mackenzie c a Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong, Victoria 3220, Australia b Department of Livestock and Fisheries, Ministry of Agriculture and Forestry, Lao PDR c Department of Microbiology and Parasitology, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia d Institute of Virology, School of Veterinary Medicine, Buenteweg 17, 30559 Hannover, Germany Received 27 November 2003; received in revised form 27 February 2004; accepted 27 February 2004 Available online 30 April 2004 Abstract The E2 genes of 21 classical swine fever viruses (CSFV) were genetically characterized and compared with reference CSF viruses. The viruses originated from CSF outbreaks that occurred in the Lao People’s Democratic Republic (Lao PDR) during 1997 though to 1999. All viruses characterized belonged to genogroup 2 and were members of subgroups 2.1 and 2.2. Results demonstrated a geographic delineation between subgroups 2.1 that was only found in the North–Central region, and subgroup 2.2 that was mostly found in the South–Central regions of Lao PDR. Although it was not possible to determine the origin of these viruses, it is probable that they may have been introduced to Lao PDR following cross-border trade. Alternat